Abstract. Maternal gene expression is an important biological process in oocyte maturation and early cleavage. To gain insights into oocyte maturation and early embryo development, we used microarray analysis to compare the gene expression profiles of germinal vesicle (GV)-and metaphase II (MII)-stage oocytes. The differences in spot intensities were normalized and grouped using the Avadis Prophetic software platform. Of the 12164 genes examined, we found 1682 genes with more highly expression in GV-stage oocytes than in MII-stage oocytes, while 1936 genes were more highly expressed in MII-stage oocytes (P<0.05). The genes were grouped on the basis of the Panther classification system according to their involvement in particular biological processes. The genes that were up-regulated in GV oocytes were more likely to be involved in protein metabolism and modification, the mitotic cell cycle, electron transport, or fertilization or belong to the microtubule/ cytoskeletal protein family. The genes specifically upregulated in the MII oocytes were more likely to be involved in DNA replication, amino acid metabolism, or expression of G protein-coupled receptors and signaling molecules. Identification of genes that are preferentially expressed at particular oocyte maturation stages provides insights into the complex gene regulatory networks that drive oocyte maturation and fertilization. Key words: Gene expression, Microarray, Mouse, Oocyte (J. Reprod. Dev. 53: [405][406][407][408][409][410][411][412][413][414][415][416][417][418] 2007) uring meiotic maturation of the mouse oocyte, dramatic qualitative changes in protein synthesis are observed as more than half of the mRNA is either deadenylated or even degraded [1,2]. The selective degradation of maternal transcripts seems to occur during both maturation of the oocyte and when the zygote is formed; with regard to the latter, selective loss of maternal transcripts marks the transfer of developmental control to the zygote genome. While the synthesis of new transcripts essentially ceases after germinal vesicle breakdown, the poly (A) tails of some classes of existing transcripts are elongated, leading to increased translation and protein levels [3]. Thus, regulation of poly (A) tail length is a major mechanism used to control the activity of maternal transcripts.Recently, the global gene expression profiles of mouse and human preimplantation embryos from the germinal vesicle (GV) oocyte through to the blastocyst have been elucidated by in vitro transcription and microarray analyses [4][5][6][7][8][9]. However, it remains unclear how the various genes that have been identified function to induce oocyte maturation and fertilization.To further understand the molecular basis of oocyte maturation and fertilization, it would be of interest to identify the genes that are specifically or p r e d o m i n a n t l y e x p r e s s e d d u r i n g o o c y t e maturation. In the present study, we first evaluated the global transcriptomes of mouse GVand MII-stage oocytes by cDNA mic...
ObjectivesWe aimed to compare tissue-specific expression profiles and biological pathways of RNA from amniocytes and amniotic fluid supernatant (AFS) from second-trimester pregnancies by using transcriptome analysis. Additionally, we wanted to explore whether cell-free RNA from AFS exhibits a unique gene expression signature that more adequately reflects the fetal developmental process than amniocyte RNA.MethodsAmniotic fluid samples were prospectively collected in the second trimester of pregnancy from euploid fetuses. Total RNA was extracted from amniocytes and AFS and hybridized to Affymetrix GeneChip Human Arrays. Significantly differentially expressed transcripts between amniocytes and AFS were obtained by using Welch’s t-test. Unsupervised hierarchical clustering was used to visualize overall expression characteristics and differences in transcripts between AFS and amniocytes. The biological functions of selected genes were analyzed using various online Gene Ontology databases.ResultsA total of 3,072 and 15,633 transcripts were detected in the second-trimester AFS and amniocytes, respectively. Hierarchical clustering revealed differential transcript expression between AFS and amniocytes. We found 353 genes that were specifically enriched in the AFS only, and tissue expression analysis showed enrichment of brain-specific genes in the AFS. Biological pathway analysis revealed that AFS-specific transcripts were mainly involved in embryonic development, cardiovascular development, and cellular morphology pathways.ConclusionThis study demonstrated differential tissue-specific gene expression profiles and biological pathways between AFS and amniocytes. The results suggested that AFS is the preferred RNA source to investigate potential biomarkers of fetal neurodevelopment.
To gain insight into early embryo development, we utilized microarray technology to compare gene expression profiles in four-cell (4C), morula (MO), and blastocyst (BL) stage embryos. Differences in spot intensities were normalized, and grouped by using Avadis Prophetic software platform (version 3.3, Strand Genomics Ltd.) and categories were based on the PANTHER and gene ontology (GO) classification system. This technique identified 622 of 7,927 genes as being more highly expressed in MO when compared to 4C (P < 0.05); similarly, we identified 654 of 9,299 genes as being more highly expressed in BL than in MO (P < 0.05). Upregulation of genes for cytoskeletal, cell adhesion, and cell junction proteins were identified in the MO as compared to the 4C stage embryos, this means they could be involved in the cell compaction necessary for the development to the MO. Genes thought to be involved in ion channels, membrane traffic, transfer/carrier proteins, and lipid metabolism were also identified as being expressed at a higher level in the BL stage embryos than in the MO. Real-time RT-PCR was performed to confirm differential expression of selected genes. The identification of the genes being expressed in here will provide insight into the complex gene regulatory networks effecting compaction and blastocoel formation.
PurposeThe purpose of this paper is to discuss the notion of “symbolic purchase” which has long been documented in the management and marketing literature. In particular, self‐congruity theory has been examined in various domains of consumer behavior. However, little attention has been paid to the area of sport. In sport business, licensed‐merchandise (i.e. team apparel) has become an increasingly important revenue source and it strengthens fan identity and brand loyalty. Therefore, based on the self‐congruity theory, the aim is to investigate the effect of self‐image congruence on sport team‐licensed merchandise evaluation and purchase.Design/methodology/approachA proposed model incorporates self‐image congruence (SIC), perceived quality, and purchase intention of team‐licensed merchandise. The reliabilities of the scaled measures were established in a pilot study (n=66). Data (n=260) for this study were collected at two professional basketball games using a convenient sampling method.FindingsThe results of the structural model indicated that the model fits the data well and constructs of self‐image congruence and perceived quality combined explained 43 per cent of the total variance in purchase intention. The findings revealed that both SIC and perceived quality had a direct positive effect on purchase intention while perceived quality also acted as a mediator between SIC and purchase intention.Research limitations/implicationsThe findings imply that SIC can be useful in predicting sport fans’ quality perception and purchase decisions on the team‐licensed merchandise. Future studies need to replicate this study in different regions and using different sports (i.e. soccer, baseball, etc.).Originality/valueThe findings extend the literature by incorporating self‐image congruence and perceived product quality in the purchase decision‐making process. This is the first known effort to apply self‐congruity theory in the spectator sport consumption context.
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