Transoral CO₂ laser microsurgery as one-stage single-modality therapy resulted in a high rate of local control and a high survival rate in selected cases of early glottic carcinoma, regardless of the location of tumor and histopathology report on the surgical margin.
Rapid and sensitive detection of influenza virus is of soaring importance to prevent further spread of infections and adequate clinical treatment. Herein, an ultrasensitive colorimetric assay called magnetic nano(e)zyme-linked immunosorbent assay (MagLISA) is suggested, in which silica-shelled magnetic nanobeads (MagNBs) and gold nanoparticles are combined to monitor influenza A virus up to femtogram per milliliter concentration. Two essential strategies for ultrasensitive sensing are designed, i.e., facile target separation by MagNBs and signal amplification by the enzymelike activity of gold nanozymes (AuNZs). The enzymelike activity was experimentally and computationally evaluated, where the catalyticity of AuNZ was tremendously stronger than that of normal biological enzymes. In the spiked test, a straightforward linearity was presented in the range of 5.0 × 10-5.0 × 10g·mL in detecting the influenza virus A (New Caledonia/20/1999) (H1N1). The detection limit is up to 5.0 × 10 g·mL only by human eyes, as well as up to 44.2 × 10 g·mL by a microplate reader, which is the lowest record to monitor influenza virus using enzyme-linked immunosorbent assay-based technology as far as we know. Clinically isolated human serum samples were successfully observed at the detection limit of 2.6 PFU·mL. This novel MagLISA demonstrates, therefore, a robust sensing platform possessing the advances of fathomable sample separation, enrichment, ultrasensitive readout, and anti-interference ability may reduce the spread of influenza virus and provide immediate clinical treatment.
BackgroundThe BRAFV600E mutation, which accounts for about 60–80% papillary thyroid carcinoma(PTC), has been identifiedas a prognostic marker for risk stratification of PTC patients. However, the BRAFV600E mutation as a prognostic marker in papillary thyroid microcarcinoma (PTMC) is unclear.MethodsWe performed a retrospective review of 101 patients who underwent surgery for PTMC. We studied the prevalence of the BRAFV600E mutation. The associations between the BRAFV600E mutation and clinicopathologic characteristics were analyzed.ResultsThe BRAFV600E mutation was observed in 72 patients (71.3%). There was no statistically significant correlation in age, gender, multifocality, extrathyroidal extension, presence of Hashimoto thyroiditis, and lymph node metastasis between the BRAFV600E mutant group and wild group.ConclusionsThe BRAFV600E mutation is not significantly associated with prognostic factors in PTMC.
Magnetic nanoparticles
have had a significant impact on a wide range of advanced applications
in the academic and industrial fields. In particular, in nanomedicine,
the nanoparticles require specific properties, including hydrophilic
behavior, uniform and tunable dimensions, and good magnetic properties,
which are still challenging to achieve by industrial-scale synthesis.
Here, we report a gram-scale synthesis of hydrophilic magnetic nanoclusters
based on a one-pot solvothermal system. Using this approach, we achieved
the nanoclusters with controlled size composed of magnetite nanocrystals
in close-packed superstructures that exhibited hydrophilicity, superparamagnetism,
high magnetization, and colloidal stability. The proposed solvothermal
method is found to be highly suitable for synthesizing industrial
quantities (gram-per-batch level) of magnetic spheres with unchanged
structural and magnetic properties. Furthermore, coating the magnetic
spheres with an additional silica layer provided further stability
and specific functionalities favorable for biological applications.
Using in vitro and in vivo studies, we successfully demonstrated both
positive and negative separation and the use of the magnetic nanoclusters
as a theragnostic nanoprobe. This scalable synthetic procedure is
expected to be highly suitable for widespread use in biomedical, energy
storage, photonics, and catalysis fields, among others.
TLM can be a relatively safe and effective salvage option for rT1 glottic cancer. However, because of its high local recurrence rate, TLM may not be generally recommended for rT2 glottic cancer.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.