Cationic lanthanide complexes are generally able to spontaneously internalize into living cells. Following our previous works based on a diMe-cyclen framework, a second generation of cationic water-soluble lanthanide complexes based on a constrained cross-bridged cyclam macrocycle functionalized with donor-π-conjugated picolinate antennas was prepared with europium(III) and ytterbium(III). Their spectroscopic properties were thoroughly investigated in various solvents and rationalized with the help of DFT calculations. A significant improvement was observed in the case of the Eu 3 + complex, while the Yb 3 + analogue conserved photophysical properties in aqueous solvent. Two-photon (2P) microscopy imaging experiments on living T24 human cancer cells confirmed the spontaneous internalization of the probes and images with good signal-to-noise ratio were obtained in the classic NIR-tovisible configuration with the Eu 3 + luminescent bioprobe and in the NIR-to-NIR with the Yb 3 + one.[a] J.
<p>Cationic
lanthanide complexes are generally able to spontaneously internalize into living
cells. Following our previous works based on diMe-cyclen framework, a second
generation of cationic water-soluble lanthanide complexes based on a
constrained cross-bridged cyclam macrocycle functionalized with donor-p-conjugated picolinate antennas has
been prepared with europium(III) and ytterbium(III). Their spectroscopic
properties were thoroughly investigated in various solvents and rationalized
with the help of DFT calculations. A significant improvement is observed in the
case of the Eu<sup>3+</sup> complex, while the Yb<sup>3+</sup> analogous conserve
an excellent brightness in aqueous solvent. Two-photon (2P) microscopy imaging
experiments on living T24 human cancer cells confirmed the spontaneous
internalization of the probes and images with good signal-to-noise have been
obtained in the classical NIR-to-visible configuration with Eu<sup>3+</sup> luminescent
bioprobe and in the NIR-to-NIR with the Yb<sup>3+</sup> one.</p>
New “all-in-one” theranostic systems, combining a magnetic resonance imaging (MRI) contrast agent with a biphotonic photodynamic therapy (2P-PDT) photosensitiser generating cytotoxic singlet oxygen, were envisaged and synthesised. They are based...
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