Recruitment of new propagules into a population can be a critical determinant of adult density. We examined recruitment dynamics in the Olympia oyster (Ostrea lurida), a species occurring almost entirely in estuaries. We investigated spatial scales of interannual synchrony across 37 sites in eight estuaries along 2,500 km of Pacific North American coastline, predicting that high vs. low recruitment years would coincide among neighboring estuaries due to shared exposure to regional oceanographic factors. Such synchrony in recruitment has been found for many marine species and some migratory estuarine species, but has never been examined across estuaries in a species that can complete its entire life cycle within the same estuary. To inform ongoing restoration efforts for Olympia oysters, which have declined in abundance in many estuaries, we also investigated predictors of recruitment failure. We found striking contrasts in absolute recruitment rate and frequency of recruitment failure among sites, estuaries, and years. Although we found a positive relationship between upwelling and recruitment, there was little evidence of synchrony in recruitment among estuaries along the coast, and only limited synchrony of sites within estuaries, suggesting recruitment rates are affected more strongly by local dynamics within estuaries than by regional oceanographic factors operating at scales encompassing multiple estuaries. This highlights the importance of local wetland and watershed management for the demography of oysters, and perhaps other species that can complete their entire life cycle within estuaries. Estuaries with more homogeneous environmental conditions had greater synchrony among sites, and this led to the potential for estuary-wide failure when all sites had no recruitment in the same year. Environmental heterogeneity within estuaries may thus buffer against estuary-wide recruitment failure, analogous to the portfolio effect for diversity. Recruitment failure was correlated with lower summer water temperature, higher winter salinity, and shorter residence time: all indicators of stronger marine influence on estuaries. Recruitment failure was also more common in estuaries with limited networks of nearby adult oysters. Large existing oyster networks are thus of high conservation value, while estuaries that lack them would benefit from restoration efforts to increase the extent and connectivity of sites supporting oysters.

Marine reserves have become increasingly valuable tools with which to manage ecosystems. These reserves consistently restore populations of top predators, often reducing availability of their favored prey. We hypothesized that such prey reduction in reserves causes protected predators to alter their attack behavior to include less palatable prey, potentially amplifying top-down effects on community structure. To test this hypothesis, we presented the relatively unpalatable sea hare Aplysia californica to freely foraging spiny lobsters Panulirus interruptus in 4 marine no-take reserves, each paired with an adjacent fished area. We found that lobsters only attacked sea hares inside reserves, where lobster density was significantly greater than that of the adjacent fished areas. Attacks on otherwise unpalatable prey exclusively in no-take reserves was likely caused by increased hunger, since in the laboratory only food-deprived lobsters attacked sea hares. These findings are the first to suggest that management involving no-take reserves may have unintended consequences on community structure that result from behavioral changes in key predators in the face of increased competition for food. We suspect that these effects may become more widely detected as reserves across the globe grow older and are researched further.

An integrative approach to investigate the species-level diversity in Oxynoe (Mollusca, Heterobranchia, Sacoglossa) revealed the existence of 11 distinct taxa. Oxynoe viridis (Pease, 1861) and Oxynoe antillarum Mörch, 1863 are redescribed; Oxynoe natalensis E. A. Smith, 1903 and Oxynoe azuropunctata Jensen, 1980 are regarded as valid. Species originally described from empty shells are regarded as nomina dubia. Seven new species are described, four from the tropical Indo-West Pacific: Oxynoe kylei, sp. nov., Oxynoe neridae, sp. nov., Oxynoe jordani, sp. nov. and Oxynoe jacksoni, sp. nov.; and two from the tropical Atlantic: Oxynoe struthioe, sp. nov. and Oxynoe ilani, sp. nov. The name Oxynoe panamensis Pilsbry & Olsson, 1943 has been applied to eastern Pacific specimens, but was introduced based on material collected from the Caribbean; therefore, the new name Oxynoe aliciae, sp. nov. is introduced for eastern Pacific specimens. Species are delineated using molecular and morphological traits, as well as algal host and reproductive biology. Results from morphological comparisons are concordant with molecular and integrative species delimitation analyses, providing robust evidence for species hypotheses. As Oxynoe is one of the few groups specialised to feed on the green algal genus Caulerpa, which includes highly invasive species, clarifying the taxonomy of Oxynoe may inform efforts to predict community response to disruptive algal invasions.

Predicting biotic resistance to highly invasive strains of "killer algae" (Caulerpa spp.) requires understanding the diversity and feeding preferences of native consumers, including sea slugs in family Oxynoidae. Past studies reported low algal host specificity for Oxynoe (6 spp.) and Lobiger (4 spp.), but these taxonomically challenging slugs may represent species complexes of unrecognized specialists that prefer different Caulerpa spp. Here, we assess global diversity of these genera by integrating gene sequences with morphological data from microscopic teeth and internal shells, the only hard parts in these soft-bodied invertebrates. Four delimitation methods applied to datasets comprising mtDNA and/or nuclear alleles yielded up to 16 species hypotheses for samples comprising five nominal taxa, including five highly divergent species in Lobiger and five in Oxynoe. Depending on the analysis, a further four to six species were recovered in the O. antillarum-viridis complex, a clade in which mitochondrial divergence was low and nuclear alleles were shared among lineages. Bayesian species delimitation using only morphological data supported most candidate species, however, and integrative analyses combining morphological and genetic data fully supported all complex members. Collectively, our findings double the recognized biodiversity in Oxynoidae, and illustrate the value of including data from traits that mediate fast-evolving ecological interactions during species delimitation. Preference for Caulerpa spp. and radular tooth characteristics covaried among newly delimited species, highlighting an unappreciated degree of host specialization and coevolution in these taxa that may help predict their role in containing outbreaks of invasive algae.