Mass spectrometry-based screening can be applied to a wide range of targets, including those intractable targets that use substrates such as lipids, fatty acids, phospholipids, steroids, prostaglandins, and other compounds not generally amenable to conventional screening techniques. The major limitation to this approach is throughput, making HTS via mass spectrometry impractical. We present a mass spectrometry-based technique and hardware for lead discovery applications. Mass spectrometry enables the design of label-free assays using biologically native substrates for a wide range of enzymatic targets. This system can be used for the direct quantification of analytes in complex reaction mixtures with typical throughputs of 4-5 s per sample. A mass spectrometry-based assay was developed to identify inhibitors of acetylcholinesterase, an enzyme with clinical importance in Alzheimer's disease. The system was used to screen a small chemical library. Several potent inhibitors were identified, and the IC(50) values of the inhibitors were determined.
Mass spectrometry-based screening can be applied to a wide range of targets, including those intractable targets that use substrates such as lipids, fatty acids, phospholipids, steroids, prostaglandins, and other compounds not generally amenable to conventional screening techniques. The major limitation to this approach is throughput, making HTS via mass spectrometry impractical. We present a mass spectrometry-based technique and hardware for lead discovery applications. Mass spectrometry enables the design of label-free assays using biologically native substrates for a wide range of enzymatic targets. This system can be used for the direct quantification of analytes in complex reaction mixtures with typical throughputs of 4-5 s per sample. A mass spectrometry-based assay was developed to identify inhibitors of acetylcholinesterase, an enzyme with clinical importance in Alzheimer's disease. The system was used to screen a small chemical library. Several potent inhibitors were identified, and the IC(50) values of the inhibitors were determined.
Soil sampling in a cereal crop near Southbridge Canterbury revealed dipteran larvae attacking Costelytra zealandica (White) pupae Approximately 50 of the pupae had larvae associated with them DNA sequencing analysis of larval specimens indicated they most likely belonged to the family Dolichopodidae Larval specimens were reared through to adults and were identified as Ostenia robusta (Hutton) (Diptera Dolichopodidae) This is the first record of the association of O robusta larvae with C zealandica pupae A general description of the adult and larva of this species is provided along with DNA sequencing data and observations on its association with C zealandica pupae The potential role of O robusta in regulating populations of C zealandica is briefly considered
Six new Orchidantha species (Lowiaceae) from Borneo, O. gigantea Škorničk. & A.Lamb, O. ismailii Škorničk. & A.Lamb, O. jiewhoei Škorničk. & A.Lamb, O. lutescens Škorničk. & A.Lamb, O. nilusii Škorničk. & A.Lamb and O. ultramafica Škorničk. & A.Lamb are described here. This brings the total number of species in the family to 34 of which 17 are endemic to Borneo. Detailed descriptions, colour plates, preliminary IUCN conservation assessments and a key to all Bornean Orchidantha species are provided.
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