[1] Results of a single-blind round-robin comparison of satellite primary productivity algorithms are presented. The goal of the round-robin exercise was to determine the accuracy of the algorithms in predicting depth-integrated primary production from information amenable to remote sensing. Twelve algorithms, developed by 10 teams, were evaluated by comparing their ability to estimate depth-integrated daily production (IP, mg C m À2 ) at 89 stations in geographically diverse provinces. Algorithms were furnished information about the surface chlorophyll concentration, temperature, photosynthetic available radiation, latitude, longitude, and day of the year. Algorithm results were then compared with IP estimates derived from 14 C uptake measurements at the same stations. Estimates from the best-performing algorithms were generally within a factor of 2 of the 14 C-derived estimates. Many algorithms had systematic biases that can possibly be eliminated by reparameterizing underlying relationships. The performance of the algorithms and degree of correlation with each other were independent of the algorithms' complexity.

The spatial and seasonal distributions of phytoplankton pigment concentration over the entire southern ocean have been studied for the first time using the coastal zone color scanner historical data set (from October 1978 through June 1986). Enhanced pigment concentrations are observed between 35øS and 55øS throughout the year, with such enhanced regions being more confined to the south in the austral summer and extending further north in the winter. North and south of the polar front, phytoplankton blooms (>1 mg/m 3) are not uniformly distributed around the circumpolar region. Instead, blooms appear to be located in regions of ice retreat (or high melt areas) such as the ScotiaSea and the Ross Sea, in relatively shallow areas (e.g., the Patagonian and the New Zealand shelves), in some regions of Ekman upwelling like the Tasman Sea, and near areas of high eddy kinetic energy such as the Agulhas retroflection. Among all features examined by regression analysis, bathymetry appears to be the one most consistently correlated with pigments (correlation coefficient being about -0.3 for the entire region). The cause of negative correlation with bathymetry is unknown but is consistent with the observed abundance of iron in shallow areas in the Antarctic region. It is also consistent with resuspension of phytoplankton cells by wind-induced mixing, especially in shallow waters. On the other hand, in the deep ocean (especially at latitudes <45øS where surface nutrients may be limiting), upwelling induced by topographic features may cause resupply of nutrients to the surface and shoaling of the subsurface chlorophyll maximum. Low pigment values are common at low latitudes and in regions of high wind stress, where deep mixing and net loss of surface pigment occur. Nutrients (phosphate, nitrate, and silicate) are found to correlate significantly with pigments when the entire southern ocean is considered, but south of 55øS the correlation is poor, probably because the Antarctic waters are not nutrient limited. Nutrients are also highly correlated with Ekman upwelling. Although cloud cover and normalized aerosol radiance are correlated to the patterns of pigment concentrations in some areas, the correlations are weak in other areas, suggesting that light and iron may not be the primary factors responsible for the spatial variability of pigment concentrations, especially during summer. Large interannual variability (>30%) in average pigment concentration over the entire region during different seasons indicates possible influence of time dependent parameters. 1.

Using field measurements and quantitative modeling, we demonstrate that red coloration of the sea surface is not associated with any particular group of phytoplankton and is strongly dependent on the physiology of the human visual system. Red or brown surface waters can be produced by high concentrations of most types of algae, colored dissolved organic matter, or suspended sediment. Even though light reflected by red tides commonly peaks in the yellow spectral region (570-580 nm), human color perception requires consideration of the entire spectrum of light relative to receptors within the human eye. The color shift from green to red is not due to any special optical properties of the algae but results from an overlap in spectral response of the eye's red and green cones (centered at 564 and 534 nm, respectively). The spectral peak in light reflected from dense algal blooms coincides with a critical hinge point in color vision (570-580 nm), where fine-scale shifts in the spectral shape of water-leaving radiance due to algal absorption and backscattering properties lead to pronounced variations in the observed color. Of the taxa considered, only Chlorophytes and Prochlorophytes lacked sufficient accessory pigments to produce a red tide. Chlorophyll fluorescence and enhanced near-infrared reflectance (the ''red edge'') contribute negligibly to the perceived color. Black water events are produced when water is highly absorbing but lacks backscattering constituents.

Environmental DNA (eDNA) can be used to identify macroorganisms and describe biodiversity, and thus has promise to supplement biological monitoring in marine ecosystems. Despite this promise, scaling sample acquisition to the spatial and temporal scales needed for effective monitoring would require prohibitively large investments in time and human resources. To address this challenge, we evaluated the efficacy of an autonomous eDNA sampling system and compare results obtained to traditional eDNA sampling methods. The autonomous sampling instrument consisted of the Environmental Sample Processor (ESP) coupled to an autonomous underwater vehicle (AUV). We tested equivalency between the ESP and traditional eDNA sampling techniques by comparing the quantification of eDNA across a broad range of taxa, from microbes (SAR11), phytoplankton (Pseudo-nitzschia spp.), and invertebrates (krill: Euphausia pacifica) to vertebrates (anchovy: Engraulis mordax). No significant differences in eDNA densities were observed between the two sample collection and filtration methods. eDNA filters collected by the ESP were preserved and stable for 21 days, the typical deployment length of the instrumentation. Finally, we demonstrated the unique capabilities of an autonomous, mobile ESP during a deployment near Monterey Bay, CA, by remotely and repeatedly sampling a water mass over 12 h. The development of a mobile ESP demonstrates the promise of utilizing eDNA measurements to observe complex biological processes in the ocean absent a human presence.

Animal-Borne Metrics Enable Acoustic Detection of Blue Whale MigrationHighlights d Acoustic monitoring reveals patterns in population-level blue whale song production d Tag-derived metrics provide behavioral context for distinct diel patterns in song d When integrated, tag and acoustic metrics reveal an acoustic signature of migration d Key to discerning timing, plasticity, and drivers of a dispersed migration

Physical-biological couplings impacting phytoplankton ecology are examined with synoptic, high-resolution observations of Monterey Bay, California. Influences of submarine canyon and shelf break topography on the physical-biological couplings are supported by 2 case studies. In the first case study, benthic-pelagic coupling was observed in southern shelf waters where a turbid plume extended from the bottom at ~60 m deep to the base of a phytoplankton layer centered at ~10 m deep. The alongshelf scale of the plume ranged from ~5 km near the bottom to ~1 km at its intersection with the phytoplankton layer. In situ and remote sensing data support the influence of Monterey Canyon on circulation forcing the benthic-pelagic coupling. In the second case study, a frontal zone and adjacent waters were rapidly surveyed over ~20 km 2 of the northern shelf. The front was associated with an isopycnal ridge/trough structure, surface slick, and frontal eddy <1 km in diameter. The magnitude and vertical location of a chlorophyll maximum layer were closely coupled with the physical environment through the frontal zone. The layer was dispersed by the isopycnal ridge and frontal eddy, and concentrated in the isopycnal trough and along the periphery of the eddy. Influence of an internal wave generated by interaction of tidal currents with the shelf break is supported by the observed surface slick, measured water velocities, and the proximity and orientation of the shelf break. Significant and persistent influences of topography on phytoplankton ecology in Monterey Bay are indicated.

Background: MBARI Long Range Autonomous Underwater Vehicle designed to collect time-series measurements (credit: Kip Evans). Left photo: The M1 mooring just after deployment (credit: Francisco Chavez). Right photo: Time series CTD deployment on R/V Rachel Carson (credit: Todd Walsh). " Time-series programs should themselves be subject to change, whenever it is necessary to do so. " .

In 8 yr (1979)(1980)(1981)(1982)(1983)(1984)(1985)(1986) of Coastal Zone Color Scanner (CZCS) imagery, we find annual enhancement of chlorophyll at the shelfbreak of the Mid-Atlantic Bight (MAB) and Georges Bank during the spring transition from well-mixed to stratified conditions. Spatial and temporal extents of enhancement vary interannually, and expression is intermittent intraannually. This feature can span the entire MAB and southern flank of Georges Bank (ϳ1,100 km) and can be expressed for as long as 10 weeks (mid-April to late June). Pigment concentrations within the feature average more than two times that of adjacent shelf and slope waters. Enhanced shelfbreak chlorophyll consistently coincided with the shelf-slope front and often extended inshore of the surface outcrop of the front a few to ϳ10 km. In all years except 1986, it coincided with seaward entrainment of shelf water by Gulf Stream warm-core rings (WCRs) or meanders. Shelfbreak chlorophyll enhancement was most pronounced during 1980. Using satellite and in situ observations, we found that during 1980, it coincided with the shelf-slope front for 10 weeks, and, unlike the spring bloom, it was dominated by the nanophytoplankton (Ͻ20 m) size fraction. During the peak of the 1980 occurrence, four WCRs simultaneously interacted with shelf water, and chlorophyll enhancement inshore of one WCR coincided with a slope-water intrusion onto the shelf. Empirical orthogonal function (EOF) decomposition of CZCS images for late March-June 1980 showed that shelfbreak enhancement was strongly pronounced in an EOF that accounted for Ͼ10% of the variance about the mean. This annual biological feature, brought to light in satellite ocean color imagery, is an important aspect of the shelf-slope ecology of the MAB and Georges Bank.