A new technique is described for measuring the adhesive strength of a gingival cell line to an agar substratum by the modification of the original "blister" test for adhesives. A cell monolayer was developed on a Petri dish with a hole in the center of the growing surface, overlayed with agar, and the system pressurized to debond the cells from the agar surface. Pressure changes were measured by a capacitance pressure transducer the output of which was measured by a strip-chart recorder. The modulus (E) of the agar overlay was determined and used in the calculation of the adhesive-bond strength (gammaalpha). The gammaalpha yield for the gingival cell line (cell-agar debond) was 48.8 ergs per cm2, and for the control (no cells) (agar-polystyrene debond) was 30.0 ergs per cm2.
The in vitro measurement of gingival cell‐bacto agar substratum adhesive‐bonding strength (γa) is described as it relates to cell‐ concentration, cell‐substratum contact time and serum concentration. These three factors are critical in obtaining reprocible γa's for gingival cell‐test substratum interactions.
Cell concentrations of less than 35 × 103 cells per cm2 gave low γa's (70–75 ergs/cm2) whereas 90–130 × 103 cells/cm2 resulted in γa's of 109–119 ergs/cm2, respectively. Maximum γa's (123 ergs/cm2) resulted after the cells had been in contact with the agar substratum for 24 hours. The adhesive‐bonding strenth after eight housrs of contact time, however, was not statistically different from the 24 housr readings and has the advantage of decreasing the test duration. Serum, and important factor for optimum adhesion measurements, produced a peak γa at the 10 % concentration.
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