Measurement of the adhesive strength of a gingival cell line to agar

Abstract

A new technique is described for measuring the adhesive strength of a gingival cell line to an agar substratum by the modification of the original "blister" test for adhesives. A cell monolayer was developed on a Petri dish with a hole in the center of the growing surface, overlayed with agar, and the system pressurized to debond the cells from the agar surface. Pressure changes were measured by a capacitance pressure transducer the output of which was measured by a strip-chart recorder. The modulus (E) of the agar overlay was determined and used in the calculation of the adhesive-bond strength (gammaalpha). The gammaalpha yield for the gingival cell line (cell-agar debond) was 48.8 ergs per cm2, and for the control (no cells) (agar-polystyrene debond) was 30.0 ergs per cm2.

Quantitation of in vitro cell adhesion to cementum, dentin and enamel

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The William's blister test for adhesion was used to determine the strength of epithelial and fibrobiast cell attachments to cementum, dentin and enamel. Differences of air pressure required (cm H^O) to debond the samples from a cell monolayer were measured and recorded on a strip chart recorder (Fletcher et al 1977).Gingival and skin-muscle cells detached at similar pressures from cementum, dentin and enamel. Higher pressures (of debond) were required with cells grown on a polystyrene surface.

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“…The William's blister test for adhesion was used to determine the strength of epithelial and fibrobiast cell attachments to cementum, dentin and enamel. Differences of air pressure required (cm H^O) to debond the samples from a cell monolayer were measured and recorded on a strip chart recorder (Fletcher et al 1977).…”