The O2 vibration of dioxygen adducts of Fe and Co model complexes of a,a,ac,a-tetrapivalamidophenylporphyrin ("picket fence" porphyrin, TpivPP) with 1-methylimidazole and 1-tritylimidazole as axial bases are reported, obtained with difference techniques between 1'602,O180, 160-.80 A continuing controversy exists over the nature of ligand bonding to hemoproteins and metalloporphyrins. While it has been well established that both iron and cobalt bind dioxygen in a bent end-on fashion (1, 2), physical measurements (3-6) and theoretical calculations (7-10) have been used to support both the spin-paired dioxygen (M"1-02) (11) and the electron transfer superoxide (MIII-02-) (12) models. Here we present infrared data on dioxygen "picket-fence" porphyrin complexes of iron and cobalt. These results strongly support the MIn-02-formalism. The present data correct the preliminary report of an oxygen vibration at 1385 cm-' (3,13) which has since proved to be an artifact* (14). We are unaware of any previous reports of Po2 in model Fe and Co porphyrins. In addition, we discuss the nature of CO binding to ferrous porphyrins and the implication this has for the role of the apoprotein in biological oxygen carriers. It is our thesis that the distal binding site in hemoproteins functions to lower the carbon monoxide affinity.MATERIALS AND METHODS Benzene (spectroquality, Baker "Photrex") solutions of the "picket fence" porphyrin complexes (3, 14), FelImesotetra(a,a,a,a-o-pivalamidophenyl)porphyrinato (TpivPP) and Coll (aa,a,a-TpivPP), 17 mM and 19 mM containing 5 and 2 equivalents of 1-tritylimidazole (NTrIm), respectively, were Abbreviations: TpivPP, meso-tetra (a,a,a,a-o-pivalamidophenyl