Kanamycin Aergibt mit Hexamethyldisilazan in siedendem Acetonitril Polytrimethylsilyl‐kanamycin A, das durch Behandlung mit N‐((2S)‐4‐Benzyloxycarbonylamino‐2‐hydroxy‐butanoyloxy)‐succinimid bei 5°C für l Std., anschließende Hydrolyse mit Wasser bei pH 2,5 und Hydrogenolyse in ca. 50%iger Ausbeute zum Amid (Ia) und in ca. 5%iger Ausbeute zum Amid (Ib) führt.
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