Variety change and genetic diversity are important means of combating crop losses from pests and diseases in modern agricultural systems. Since the Green Revolution, genetic diversity among wheat varieties released in India has increased but variety change on farms continues to be slow. In this article, we define and summarise indices of variety change and genetic diversity for the wheat varieties released and grown in Indian Punjab during the post‐Green Revolution period. We evaluate the effect of each index on technical efficiency with a Cobb‐Douglas yield model after testing for exogeneity. Findings support the hypothesis that slow variety change has offset the positive productivity effects of diversifying the genetic base in wheat breeding during the post‐Green Revolution period. Policies that speed the rate of variety change and contribute to a more equitable spatial distribution of modern varieties could support wheat productivity in the Punjab of India, reinforcing plant breeding successes.
Administration of ground-up tick tissue stabilate (0.75 tick equivalent) by the subcutaneous route to crossbred calves aged 1 week to 1 month led to the development of acute theileriosis. Haematological studies revealed significant progressive decreases in haemoglobin concentration, packed cell volume and red blood cell count, whereas the total leukocyte count showed an initial non-significant leukocytosis followed by a significant leukopenia. Analysis of serum revealed significant increases in levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatinine kinase and gamma-glutamyltransferase, and in the concentrations of uric acid, blood urea nitrogen and bilirubin. The concentrations of total protein, albumin, glucose, cholesterol and calcium showed non-significant decreases, while phosphorus decreased significantly during the terminal stages of the disease.
The protein contents in 72 diverse accessions of lentil ranged from 10.5 to 23.7 % with an average of 18.7 % in varieties/breeding lines, 14.5 to 27.1 % with an average of 22.4 % in landraces of Mediterranean origin and 18.1 to 32.7 % with an average of 22.6 % in wild species. Significantly high protein content (32.7 %) was recorded in accession ILWL 47 belonging to Lens ervoides. The coefficient of variation and standard deviation in Mediterranean landraces (18.1 %) showed high protein contents as compared to varieties/breeding lines (16.9 %) and wild species (17.8 %). As per the Jaccard's similarity coefficient based on protein content 72 accessions were grouped into two main clusters. Cluster I possessed a solitary accession ILWL 47 while remaining accessions were grouped in cluster II, which was subdivided into sub cluster IIa with 48 genotypes and IIb comprising of 29 accessions of varieties/breeding lines, 16 of wild species and 3 landraces. The results indicated that Indian varieties/breeding lines are significantly different from Mediterranean landraces and wild species in respect of protein contents.
Subcutaneous inoculation of 1 ml of ground Theileria annulata tick tissue stabilate (0.75 tick equivalent) into crossbred calves (n = 6, average age 53 days) resulted in the development of acute theileriosis. The percentage parasitaemia was 71.7% +/- 3.3% on day 20 after inoculation. Macroschizonts were observed in lymphocytes and monocytes. Phagocytosed schizonts were observed in neutrophils, along with cytoplasmic vacuolation in monocytes and neutrophils. There was progressive decrease (p < 0.05) in the haemoglobin and packed cell volume, along with a marked reticulocytosis. Serum analysis revealed a decrease (p <0.05) in the concentrations of calcium, cholesterol and triglycerides, while there was an increase (p < 0.05) in the concentrations of blood urea nitrogen as compared to day 0 values. The total serum proteins, albumin and serum immunoglobulin concentrations and the albumin-to-immunoglobulin ratio showed marked decreases (p<0.05). Coagulopathies included thrombocytopenia and an increased prothrombin time, along with a non-significant increase in the bleeding time and activated partial thromboplastin time during the terminal stages of the disease. There was an increase in the osmotic fragility of erythrocytes during the disease. Morphological alterations in the erythrocytes were observed with the developing parasitaemia.
An outbreak of bacillary haemoglobinuria was recorded in 60 out of 110 sheep in Ludhiana, Punjab, India. The condition was clinically characterised by fever, haemoglobinuria, constipation, weakness of hind quarters followed by recumbency, respiratory distress and death in 16 sheep. Haematological studies revealed moderate to severe degrees of anaemia associated with leucocytosis. Plasma gamma-glutamyl transferase, alkaline phosphatase and creatinine phosphokinase activities were significantly higher in haemoglobinuric sheep. Babesiosis and copper poisoning were ruled out on stained blood film examination and from blood mineral profiles, respectively. Post-mortem examination of affected sheep revealed no gross changes. Pure cultures of Clostridium haemolyticum isolated from heart blood, liver, kidney and spleen of freshly killed sheep confirmed the disease. Parenteral administration of procaine penicillin was effective in the treatment of affected sheep.
An experiment on the use of farmyard manure and biofertilizer along with application of chemical phosphorus was conducted to assess the impact of differential doses of phosphorus, farmyard manure and consortium biofertilizer application on the development, yield and phosphorus uptake during the year 2018 and 2019. The impact of different treatments was recorded on the plant height, dry matter partition, yield and yield attributes, phosphorus uptake and soil phosphorus availability using standard methods. The data revealed significant improvement in yield, yield attributes, phosphorus uptake and soil phosphorus availability. The integration of farmyard manure and biofertilizer with 60 kg ha-1 SSP (single superphosphate) has improved the black gram yield by 7.4% and 3.28% respectively over the use of 60 SSP alone. The phosphorus uptake in black gram with application of Farm yard manure and biofertilizer along with 60 kg ha-1 SSP has improved the uptake by 7.18% and 2.51% respectively over the use of 60 kg ha-1 SSP alone. The results highlight the need of integrated application of farm yard manure, biofertilizer for sustainable production of black gram in the region.
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