The fate of the cells of the stratified squamous epithelium of the esophagus was investigated radioautographically in young adult rats at various time intervals after a single injection of thymidine-H3.Soon after injection, labeled cells appeared i n the basal layer of the epithelium (stratum basale). During the following 12 hours, the labeled cells completed DNA synthesis and mitosis, and mostly remained in the basal layer. After 12 hours, however, the labeled cells arising from the mitoses were transferred to the spinous layer (stratum spinosum) at the rate of 1.2% per hour. The respective fates of the two daughter cells of a mitosis were then examined using two-dimensional maps showing the location of the labeled nuclei at 24 and 48 hours after injection. It was assumed that any two nuclei located side by side and overlaid by a similar number of grains are the two daughter cells of a mitosis. Such pairs of labeled daughter cells fell into three categories: (1) Basal puirs, composed of two basal cells; ( 2 ) Outgoing pairs, composed of two spinous (or granular) cells; and (3) Mixed pairs, composed of one basal and one spinous (or granular) cell. Since three possibilities were encountered at the two time intervals, the mitoses could not be differential (in which case the pairs of daughter cells would consist of a cell remaining in the basal layer and another migrating to the spinous layer, that is, all pairs would be "mixed"). Instead, the frequency of the three types of pairs was such as to indicate that the transfer of a basal cell to the spinous layer is a chance event which can affect any basal cell (except those undergoing DNA synthesis or mitosis). Accordingly, the transfer of either or both daughter cells of a mitosis would also be due to chance.The transfer of a cell out of the basal layer is a critical step in the life of the cell, since it precludes further division and appears to trigger differentiation.

Intramyocardial transplantation of BMMC in dilated cardiomyopathy cases is feasible and safe. There were early improvements in symptoms and LV performance. Medium-term evaluation revealed regression of LV function, although maintaining improved functional class.

To assess the usefulness of cord blood tests in diagnosing ABO-haemolytic disease of the newborn (ABO-HDN), 132 term, adequate for gestational age (AGA) neonates were evaluated. The tests studied and their significant results were: quantitative elution test (greater than or equal to 1/16), direct Coombs test (positive), bilirubin concentration (greater than or equal to 4 mg/dl). In none of the 56 O+ newborn infants delivered by O+ women were the results of any test positive. Of the 76 A+ and B+ newborn infants delivered by O+ women, 17 (22%) developed ABO-HDN. When the combined result of any two tests was positive, the sensitivity, the specificity and the positive predictive accuracy for the diagnosis of ABO-HDN was higher than for any one of the isolated tests. The probability that ABO-HDN was present when the results of at least two cord blood tests were positive was 70%, and the probability that ABO-HDN was not present when less than two cord blood tests gave positive results was 93%. It is suggested that the combination of quantitative elution test, bilirubin concentration and direct Coombs test in the cord blood is useful for an early diagnosis of ABO-HDN.

In 82 patients with B‐chronic lymphocytic leukemia, the correlations between the histopathologic bone marrow patterns (interstitial, nodular, mixed, and diffuse), serum immunoglobulin (Ig), and blood B‐lymphocyte levels and T‐lymphocyte levels have been analyzed. The most commonly lowered Ig class was IgM (43% of cases) followed by IgA (35%), and IgG (10%). There was a trend to a more frequent decrease of Ig levels when “advanced” bone marrow patterns (mixed + diffuse) were compared with the earlier ones (interstitial + nodular). The frequency of such a decrease was statistically significant for IgA class (P < 0.01). The absolute number of blood T‐lymphocytes was increased in the diffuse pattern (P < 0.01).

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mente (p <0,001) o uso de CHs, diminuindo os riscos aos quais os pacientes poderiam estar expostos. (p < 0.001). Rev. bras. hematol. hemoter. 2008; 30(4):333-334. Abstract This study describes the impact of the implantation of a document of responsibility that should be followed by doctors who do not comply with the guidelines of Hospital de Clínicas when prescribing packed red blood cells. Data analysis shows that the compulsory nature of the responsibility terms significantly decreases the number of packed red blood cells transfused in opposition to the Institution guidelines