The chromatographic analysis of ethanolic extract from N a e m a t o 1 o m a f a s c i c u 1 a r e fruitbodies indicated 20 nitrogen compounds (4 liquid amines, 4 volatile amines, 10 amino acids, 2 purines) 5 sugars, 4 sterols. Ergosterol, ergosterol peroxide, cerevisterol were separated by column chromatography and crystallized.Naematoloma fasciculare HUDS. ex Fr. P. KARST* a representative of xylophilous Basidiomycetes beIongs to the family Strophariaceae [9, 171. Ethanolic extract of N. fasciculare shows diuretic and parasymphathetic action in rats after ip administration [15]. Herbich et al. [6] reported that the fatal poisonings of men with fruitbodies of N. fasciculare were connected with necrosis of the liver, damage of the cardiac muscle and brain cells. The previously described*:-investigations on mycelial cultures (surface and submerged) obtained from the explantats of N. fasciculare fruitbodies and analyzed compounds biosynthesized in vitro.The aim of the present study was a phytochemical analysis of the constituents occuring in ethanolic extract of N. fasciculare fruitbodies collected in the natural habitats. ExperimentalGeneral: The infrared spectra were taken in KBr pellets on a Digilap. Mod. FTS-20 spectrometer. The mass spectra were measured with a LKB 9000s in the Regional Laboratory of Physicochemical Analysis and Structural Research in Krak6w. The melting points were determined on a Boetius hot stage microscope and are uncorrected. * synonym: Hypholorna fasciculare (FR.) QUEL. :' * J. DIAK: POI.Abreviations used: Be = benzene, n-BuOH = n-butanol, EtOAc = ethyl acetate, He = heksane, HOAc = acetic acid, M,Co = acetone, MeCOEt = ethylmethyl ketone, MeOH = methanol, PhOH = phenol, iso-PrOH = iso-propanol, Pyr = pyridine.Plant material: The well developed (open hats) fruitbodies of N. fasciculare were collected in the natural habitats in 1973-1975.Extraction methods: The fresh fruitbodies were washed in runnig water and homogenized with ethanol. After centrifugation of the precipitate, ethanolic extract was analyzed acc. to the scheme in Fig. 1.Paper chromatography: For separation of nitrogen compounds and sugars,
From the aerial parts of Penstemon diffusus DOUGL. (Scrophulariaceae) have been isolated three iridoid glucosides: penstemide (I), aucubin (V), and loganin (VIII). The identity of the compounds and their derivatives has been confirmed by spectroscopic data.
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