Understanding how grape (Vitis L.) vines store nutrients in permanent tissues, how much nutrient vines take up from the soil, and how nutrients are partitioned and redistributed throughout the plant is critical to the development of sound nutrient management practices. This study investigated the seasonal patterns of macronutrient uptake and redistribution in whole ‘Concord’ grape (Vitis labruscana Bailey) vines. The study was conducted in a 42-year-old own-rooted ‘Concord’ vineyard. The site was a furrow-irrigated fine sandy loam. In 2006 and 2007, four vines were excavated at winter pruning, budbreak, three- to four-leaf stage, bloom, veraison, harvest, and postharvest. Each vine was separated into different organs, dried, and weighed to determine biomass, and then ground and analyzed for C, N, P, K, Ca, and Mg. The results showed that the seasonal dynamics of nutrient contents shared a consistent pattern: translocation of nutrients from woody tissues to actively growing organs at the beginning of the season; nutrient uptake from bloom to veraison (P and Mg in 2006), bloom to harvest (N, P, K, and Ca), or veraison to harvest (P and Mg in 2007); and nutrient movement to woody tissues occurring after veraison until leaf fall with no further nutrient uptake. There was a very high accumulation of Ca in permanent structures of the vine, reflecting the high Ca and CaCO3 found in the soils of the region. As a result, the vines had a higher Ca content than all other nutrients throughout the growing season, which is different from findings in other growing areas.