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Aims-To identify parietal cells in the upper gastrointestinal tract by an immunoperoxidase method, using commercially available monoclonal antibodies.Methods-Routine surgical biopsy specimens of gastric body mucosa were examined using the avidin-biotin peroxidase method with the monoclonal antibodies HMFG-l and HMFG-2 to identify parietal cells. Double immunoperoxidase labelling with HKl2.18, a well characterised monoclonal antibody directed against an epitope on the a (catalytic) subunit of H+ translocating, K+ stimulated adenosine triphosphatase (H,K-ATPase), was used to confirm that HMFG-1 and -2 stained parietal cells. Results-HMFG-1 and HMFG-2 showed consistent parietal cell staining patterns in the gastric body mucosa. HMFG-2 gave a more intense staining pattern of the secretory canaliculi. This was confirmed by double immunolabelling with HK12.18. Conclusions-HMFG monoclonal antibodies are reconumended as highly specific markers of human gastric parietal cells. (J Clin Pathol 1995;48:832-834)

Aim-To investigate the prevalence, and relation to Helicobacter pyloni, of parietal

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