Identification of parietal cells in gastric body mucosa with HMFG-2 monoclonal antibody.

Walker, M M; Smolka, Adam J.; Waller, JM; Evans, Doyle J.

doi:10.1136/jcp.48.9.832

Cited by 12 publications

(6 citation statements)

References 8 publications

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“…This antibody has recently been described as a specific marker of parietal cells because of its reactivity with antigen in canalicular membranes 16. Following serial titration studies to determine optimal dilutions, COX-1, COX-2, and HMFG were routinely used at 1:500, 1:500, and 1:75 dilutions, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The gastric mucosal biopsy samples, completely denuded of epithelial cells, were subsequently cultured at 37°C in RPMI 1640 (Gibco/BRL) containing 10% fetal calf serum (Gibco/BRL) in 60 mm tissue culture dishes (Costar Corp., Cambridge, Massachusetts, USA) for 24 hours 16. The cells that had migrated out of the biopsy sample and were in suspension or on the bottom of the dishes were collected by vigorous pipetting and centrifugation following incubation at 4°C for one hour.…”

Section: Methodsmentioning

confidence: 99%

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Cyclooxygenase (COX) 1 and 2 in normal, inflamed, and ulcerated human gastric mucosa

Jackson

Mahida

et al. 2000

Gut

176

135

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Background and aims-Constitutive cyclooxygenase (COX) 1 is believed to mediate prostaglandin dependent gastric protection. However, gastric mucosa contains cells capable of expressing inducible COX-2. We therefore investigated COX-1 and COX-2 expression, localisation, and activity in normal and abnormal human gastric mucosa. Methods-COX-1 and COX-2 distribution was investigated by light and electron microscopic immunohistochemistry and by western blot analysis, and their contribution to prostaglandin (PG)E 2 synthesis using selective enzyme inhibitors. Results-There was strong parietal cell COX-1 and COX-2 immunoreactivity in all sections and isolated cells, with macrophage and myofibroblast reactivity in some sections. Immunostaining was specifically abolished by antigen absorption. Western blot analysis confirmed COX-1 and 2 expression. COX-1 and COX-2 immunostaining was increased in Helicobacter pylori gastritis, particularly the mid glandular zone and lamina propria inflammatory cells. This was associated with increased ex vivo PGE 2 synthesis (62.4 (13.5) pg/mg v 36.3 (15.5) pg/mg in uninflamed mucosa; p=0.017) which was significantly inhibited by COX-1 but not COX-2 inhibition. Increased COX-2 immunostaining in macrophages, endothelial cells, and myofibroblasts (with reduced epithelial expression) was seen at the rim of ulcers. Conclusion-COX-2, as well as COX-1, is expressed by normal human gastric mucosa and is increased at the rim of ulcers. Although both are increased with H pylori, COX-1 contributes more than COX-2 to gastric PGE 2 production.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Cyclooxygenase (COX) 1 and 2 in normal, inflamed, and ulcerated human gastric mucosa

Jackson

Mahida

et al. 2000

Gut

176

135

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“…MUC1 is widely expressed in mucous cells of the surface epithelium of the antrum, and is focally expressed in oxyntic glands of the body and pyloric glands of the antrum (Burchell et al 1987;Girling et al 1989;Ho et al 1993Ho et al ,1995Walker et al 1995;Reis et al 1998a). MUC5AC is highly expressed in foveolar cells of the body and the antrum (De Bolós et al 1995;Ho et al 1995;Byrd et al 1997;Reis et al 1997), whereas MUC6 is expressed in mucopeptic cells of the neck region of the body and in pyloric glands of the antrum (De Bolós et al 1995;Ho et al 1995;Byrd et al 1997;Bartman et al 1998;Reis et al 1999).…”

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confidence: 99%

Immunohistochemical Study of the Expression of MUC6 Mucin and Co-expression of Other Secreted Mucins (MUC5AC and MUC2) in Human Gastric Carcinomas

Reis

David

Carvalho

et al. 2000

J Histochem Cytochem.

147

129

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SUMMARYTo investigate the expression of MUC6 mucin in gastric carcinomas, we generated a novel monoclonal antibody (MAb CLH5) using an MUC6 synthetic peptide. MAb CLH5 reacted exclusively with the MUC6 peptide and with native and deglycosylated mucin extracts from gastric tissues. MAb CLH5 immunoreactivity was observed in normal gastric mucosa restricted to pyloric glands of the antrum and mucopeptic cells of the neck zone of the body region. In a series of 104 gastric carcinomas, 31 (29.8%) were immunoreactive for MUC6. The expression of MUC6 was not associated with histomorphological type or with clinicopathological features of the carcinomas. Analysis of the co-expression of MUC6 with other secreted mucins (MUC5AC and MUC2) in 20 gastric carcinomas revealed that different mucin core proteins are co-expressed in 55% of the cases. MUC6 was co-expressed and co-localized with MUC5AC in 45% and with MUC2 in 5% of the cases. Expression of MUC2 alone was observed in 25% of the cases. All carcinomas expressing MUC2 mucin in more than 50% of the cells were of the mucinous type according to the WHO classification. The co-expression of mucins was independent of the histomorphological type and stage of the tumors. In conclusion, we observed, using a novel well-characterized MAb, that MUC6 is a good marker of mucopeptic cell differentiation and is expressed in 30% of gastric carcinomas, independent of the clinicopathological features of the cases. Furthermore, we found that co-expression and co-localization of mucins in gastric carcinomas is independent of histomorphology and staging. Finally, we observed that intestinal mucin MUC2 is expressed as the most prominent mucin of the mucins tested in mucinous-type gastric carcinomas.

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“…1 Such cells can be identified utilizing antiproton pump antibodies, MUC-1 immunohistochemistry, and a modified Giemsa stain. [1][2][3] Exaggerated vacuolation of parietal cells mimicking signet ring cell carcinoma, however, has rarely been reported. To date, only 1 previous paper describes 2 similar cases.…”

Section: Discussionmentioning

confidence: 99%

Fundic Gland Polyp With Parietal Cell Vacuolation Mimicking Signet Ring Cell Carcinoma

Coyne

Thampy

2021

Int J Surg Pathol

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Identification of parietal cells in gastric body mucosa with HMFG-2 monoclonal antibody.

Cited by 12 publications

References 8 publications

Cyclooxygenase (COX) 1 and 2 in normal, inflamed, and ulcerated human gastric mucosa

Cyclooxygenase (COX) 1 and 2 in normal, inflamed, and ulcerated human gastric mucosa

Immunohistochemical Study of the Expression of MUC6 Mucin and Co-expression of Other Secreted Mucins (MUC5AC and MUC2) in Human Gastric Carcinomas

Fundic Gland Polyp With Parietal Cell Vacuolation Mimicking Signet Ring Cell Carcinoma

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