Piperine is the main active component of Piper longum L., which is also the main component of anti-sciatica Mongolian medicine Naru Sanwei pill. It has many pharmacological activities such as anti-inflammatory and immune regulation. This paper aims to preliminarily explore the potential mechanism of piperine in the treatment of sciatica through network pharmacology and molecular docking. TCMSP, ETCM database and literature mining were used to collect the active compounds of Piper longum L. Swiss TargetPrediction and SuperPred server were used to find the targets of compounds. At the same time, CTD database was used to collect the targets of sciatica. Then the above targets were compared and analyzed to select the targets of anti-sciatica in Piper longum L. The Go (gene ontology) annotation and KEGG pathway of the targets were enriched and analyzed by Metascape database platform. The molecular docking between the effective components and the targets was verified by Autodock. After that, the sciatica model of rats was established and treated with piperine. The expression level of inflammatory factors and proteins in the serum and tissues of rat sciatic nerve were detected by ELISA and Western blot. HE staining and immunohistochemistry were carried out on the sciatica tissues of rats. The results showed that Piper longum L. can regulate the development of sciatica and affect the expressions of PPARG and NF-kB1 through its active ingredient piperine, and there is endogenous interaction between PPARG and NF-kB1.Jiu-wang Yu and Hong-wei Yuan have contributed equally to this work.
The purpose of this paper is to explore the possible mechanisms of anti-inflammatory and scar repair by Mongolian horse oil. We used TCM database and literature mining to collect active compounds of horse oil and used Swiss TargetPrediction and SuperPred server to find targets of compounds. Anti-inflammatory drug targets were collected through the CTD database. Go annotation of targets and KEGG pathway were enriched and analyzed through Metascape database platform. Molecular docking between active ingredients and targets was verified by AutoDock software. Metascape analysis revealed that the key candidate targets were significantly enriched in a number of pathways associated with inflammatory pathology. The results of molecular docking showed that oleic acid, a major component of animals oil, could influence the regulatory functions of TNF, NGF, IL6, IL1B, Jun, and CDK1. This suggests that animals oil can regulate the development of inflammation through its active ingredient, oleic acid, and can influence the expression of multiple signaling pathways, with theoretical endogenous interactions with TNF, NGF, IL6, IL1B, JUN, and CDK1 proteins.
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