Erosion of needle electrodes in the pulsed corona discharge in water with a pulse energy of ∼ 2÷; 3 J was investigated in dependence on the electrode material (platinum, tungsten and stainless-steel) and the solution conductivity (100 and 500 µS/cm). Erosion of electrodes remarkable increased with the higher solution conductivity for all three tested metals. The highest erosion rates were determined for tungsten while platinum was the least eroded material. In addition to the dominant melting effect, release of anode material by the electrolysis significantly contributed to the total erosion of needle electrodes. The highest contribution of electrolysis was determined for stainless-steel electrodes that released up to 40-50 % of eroded metal in the form of iron ions. Peculiar protrusions were observed on the surface of eroded tungsten electrodes.

Siderophores play important roles in microbial iron piracy, and are applied as infectious disease biomarkers and novel pharmaceutical drugs. Inductively coupled plasma and molecular mass spectrometry (ICP-MS) combined with high resolution separations allow characterization of siderophores in complex samples taking advantages of mass defect data filtering, tandem mass spectrometry, and iron-containing compound quantitation. The enrichment approaches used in siderophore analysis and current ICP-MS technologies are reviewed. The recent tools for fast dereplication of secondary metabolites and their databases are reported. This review on siderophores is concluded with their recent medical, biochemical, geochemical, and agricultural applications in mass spectrometry context.

Abstract. Nonribosomal peptides have a wide range of biological and medical applications. Their identification by tandem mass spectrometry remains a challenging task. A new open-source de novo peptide identification engine CycloBranch was developed and successfully applied in identification or detailed characterization of 11 linear, cyclic, branched, and branch-cyclic peptides. CycloBranch is based on annotated building block databases the size of which is defined by the user according to ribosomal or nonribosomal peptide origin. The current number of involved nonisobaric and isobaric building blocks is 287 and 521, respectively. Contrary to all other peptide sequencing tools utilizing either peptide libraries or peptide fragment libraries, CycloBranch represents a true de novo sequencing engine developed for accurate mass spectrometric data. It is a stand-alone and cross-platform application with a graphical and userfriendly interface; it supports mzML, mzXML, mgf, txt, and baf file formats and can be run in parallel on multiple threads. It can be downloaded for free from http://ms.biomed.cas.cz/cyclobranch/, where the User's manual and video tutorials can be found.

Current mass spectrometry, nuclear magnetic resonance spectroscopy, and X-ray diffraction are presented as structure elucidation tools for analytical chemistry of natural products. Discovering new molecular entities combined with dereplication of known organic compounds represent prerequisites for biological assays and for respective applications as pharmaceuticals or molecular markers. Liquid chromatography is briefly addressed with respect to its use in mass spectrometry- and nuclear magnetic resonance-based metabolomics studies.

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Optical systems with variable optical characteristics (zoom lenses) find broader applications in practice nowadays and methods for their design are constantly developed and improved. We describe a relatively simple method of the design of zoom lenses using the third-order aberration theory. It presents one of the possible approaches of obtaining the Seidel aberration coefficients of individual members of a zoom lens. The advantage of this method is that Seidel aberration coefficients of individual elements of a given optical system can be obtained simply by solving of a set of linear equations. By using these coefficients, one can determine residual aberrations of the optical system without detailed knowledge about the structure of its individual elements. Furthermore, we can determine construction parameters of the optical system, i.e., radii of curvature and thicknesses of individual elements of a given optical system. The proposed method makes it possible to determine which elements of the optical system can be designed as simple lenses and which elements must have a more complicated design, e.g., doublets or triplets.

Noncontact optical metrology based on the chromatic confocal principle is becoming increasingly important for fast and accurate measurements of surface topography, distance, and layer thickness in engineering and industry. These sensors are based on the wavelength dependence of longitudinal chromatic aberration of optical systems, and the distance or thickness of the measured sample is coded into spectral information. We provide a theoretical analysis of a problem of the thickness measurement of transparent samples (glass plane-parallel plates or lenses) with respect to material dispersion. Our work deals with a description and analysis of induced measurement errors in the cases of measurement of the thickness of a plane-parallel plate and the central thickness of a lens. Relations are derived for a quantitative evaluation of these errors and a method is presented for minimizing the influence of these errors on the accuracy of measurement.

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