Characterization of microbial siderophores by mass spectrometry

Pluháček, Tomáš; Lemr, Karel; Ghosh, Dipankar; Milde, David; Novák, Jiří; Havlı́ček, Vladimı́r

doi:10.1002/mas.21461

Cited by 63 publications

(47 citation statements)

References 104 publications

(57 reference statements)

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“…For example, siderophores, which are important natural iron chelators, represent a novel class of antibiotics with considerable therapeutic potential [53]. Chitinase acts as an antagonist against fungi by inhibiting spore germination and germ-tube elongation [32].…”

Section: Discussionmentioning

confidence: 99%

Antifungal Activity of Isolated Bacillus amyloliquefaciens SYBC H47 for the Biocontrol of Peach Gummosis

Yanzhou

Wei

et al. 2016

PLoS ONE

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The gummosis disease is caused by Botryosphaeria dothidea (Moug. ex. Fr) Ces. et de Not., and it is one of the most important diseases of stone fruits worldwide. The use of biocontrol as an alternative approach to synthetic chemical fungicides has aroused general concern about how to control plant diseases that are caused by phytopathogens. The aim of this study is to isolate Bacillus strains from raw honeys with the capacity to inhibit B. dothidea and to explore the mechanisms by which they could be used in the biocontrol of peach gummosis. Bacillus amyloliquefaciens SYBC H47 was isolated and identified on the basis of its physiological and biochemical characteristics and its 16S rRNA and gyrB gene sequences. The cell suspension and the cell-free supernatant of its culture showed significant antifungal activity against Aspergillus niger, Mucor racemosus, Fusarium oxysporum, Penicillium citrinum, and Candida albicans by agar-diffusion assays. The primary antifungal substances were bacillomycin L, fengycin, and surfactin, which were analyzed by HPLC LC/ESI-MS/MS. Bacillomycin L showed the best inhibitory effect against conidial germination of B. dothidea, followed by fengycin and surfactin. Surfactin had limited effects on mycelial growth, contrary to those of bacillomycin L and fengycin. However, a mixture of the three lipopeptides had a synergistic effect that disrupted the structure of the conidia and mycelia. In order to reduce the production cost, the use of waste frying peanut oil and soy oil as the sole carbon source increased the lipopeptide yield levels by approximately 17% (2.42 g/L) and 110% (4.35 g/L), respectively. In a field trial, the decreases in the infected gummosis rate (IGR) and the disease severity index (DSI) through cell suspension treatments were 20% and 57.5% (in 2014), respectively, and 40% and 57.5% (in 2015), respectively, in comparison with the control. In conclusion, B. amyloliquefaciens SYBC H47 could inhibit the germination of conidia and the growth of mycelia from B. dothidea; therefore, this strain behaves as a potential biocontrol agent against the gummosis disease.

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Section: Discussionmentioning

confidence: 99%

Antifungal Activity of Isolated Bacillus amyloliquefaciens SYBC H47 for the Biocontrol of Peach Gummosis

Yanzhou

Wei

et al. 2016

PLoS ONE

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“…To circumvent these problems, pre-concentration of samples with solid-phase extraction and HPLC is used to separate siderophores (Pluhacek et al, 2016). Mass spectrometry is the most important and promising method to discover and characterize siderophores and associated receptors in fully sequenced microorganisms (Lv et al, 2014;Mathavan et al, 2014;Podkowa et al, 2014) (Fig.…”

Section: Innovative Applications Of Siderophores In Deciphering Tmentioning

confidence: 99%

“…Specifically, different types of mass spectrometry, such as inductively coupled plasma-mass spectrometry (ICP-MS), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), electrospray-ionization mass spectrometry (ESI-MS), affinity selection-mass spectrometry (ASMS), laser ablation electrospray ionization-mass spectrometry (LAESI-MS), and mass spectrometry imaging (MSI) techniques (MALDI-MS imaging and DESI-MS imaging), have been broadly used to identify siderophores (Pluhacek et al, 2016;Su et al, 2016;Hagan et al, 2017;Walker et al, 2017). In short, mass spectrometry and associated technologies are emerging as novel tools for the annotation of chemical, biological, and biomedical applications of pathogen-associated siderophores.…”

Section: Innovative Applications Of Siderophores In Deciphering Tmentioning

confidence: 99%

Mass spectrometry and associated technologies delineate the advantageously biomedical capacity of siderophores in different pathogenic contexts

Luo

Guo

et al. 2018

Mass Spectrometry Reviews

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Siderophores are chemically diverse small molecules produced by microorganisms for chelation of irons to maintain their survival and govern some important biological functions, especially those cause that infections in hosts. Still, siderophores can offer new insight into a better understanding of the diagnosis and treatments of infectious diseases from the siderophore biosynthesis and regulation perspective. Thus, this review aims to summarize the biomedical value and applicability of siderophores in pathogenic contexts by briefly reviewing mass spectrometry (MS)-based chemical biology and translational applications that involve diagnosis, pathogenesis, and therapeutic discovery for a variety of infectious conditions caused by different pathogens. We highlight the advantages and disadvantages of siderophore discovery and applications in pathogenic contexts. Finally, we propose a panel of new and promising strategy as precision-modification metabolomics method, to rapidly advance the discovery of and translational innovations pertaining to these value compounds in broad biomedical niches. © 2018 Wiley Periodicals, Inc. Mass Spec Rev XX:XX-XX, 2018.

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“…Pyoverdines are a large family of nonribosomal peptides and siderophores (ie, iron‐chelating compounds) produced by pseudomonads . Using pyoverdine as an example, in this work, we show how our open‐source tool CycloBranch can also be used to reveal the mutual ratio of 2 siderophores in a mixture.…”

Section: Introductionmentioning

confidence: 99%

“…16 Pyoverdines are a large family of nonribosomal peptides and siderophores (ie, iron-chelating compounds) produced by pseudomonads. [17][18][19] Using pyoverdine as an example, in this work, we show how our open-source tool CycloBranch can also be used to reveal the mutual ratio of 2 siderophores in a mixture. For electrospray ionization in positive ion mode, the respective pyoverdines D and E were detected as protonated molecules at m/z 1334.59713 and 1333.61310, respectively.…”

mentioning

confidence: 99%

CycloBranch: An open tool for fine isotope structures in conventional and product ion mass spectra

et al. 2018

Self Cite

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Within the growing community of Fourier transform mass spectrometry users, the identification of fine isotope structure has become an indispensable method for molecular formula determination. In this work, the fine isotope envelopes for accessing the mutual ratio of 2 closely related pyoverdines in a mixture were used. Bacterial siderophores pyoverdines D and E cannot be easily separated via liquid chromatography-mass spectrometry because their structures differ in (de)amidation at the respective chromophore parts only. Their mutual ratio was determined in a mixture via nuclear magnetic resonance spectroscopy and semiquantitative mass spectrometry using our open-source software CycloBranch, which represents a genuine free tool supporting the determination of fine isotope structures in both conventional and product ion mass spectra. Native Bruker, Thermo, and Waters data formats are supported in addition to XML and plain text formats.

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Characterization of microbial siderophores by mass spectrometry

Cited by 63 publications

References 104 publications

Antifungal Activity of Isolated Bacillus amyloliquefaciens SYBC H47 for the Biocontrol of Peach Gummosis

Antifungal Activity of Isolated Bacillus amyloliquefaciens SYBC H47 for the Biocontrol of Peach Gummosis

Mass spectrometry and associated technologies delineate the advantageously biomedical capacity of siderophores in different pathogenic contexts

CycloBranch: An open tool for fine isotope structures in conventional and product ion mass spectra

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