An experimental study of turbulent wave–current boundary layer flows is performed using a state-of-the-art oscillating water tunnel (OWT) for flow generation and a particle image velocimetry system for velocity measurements. The current velocity profiles in the presence of sinusoidal waves indicate a two-log-profile structure suggested by the widely-used Grant–Madsen model. However, for weak currents in the presence of nonlinear waves, the two-log-profile structure is contaminated or even totally obliterated by the boundary layer streaming which is produced by the asymmetry of turbulence in successive half-periods of nonlinear waves. To interpret experimental results, a semi-analytical model which adopts a rigorous way to account for a time-varying turbulent eddy viscosity is developed. The model can accurately predict turbulence asymmetry streaming, which leads to successful predictions of the mean velocity embedded in nonlinear-wave tests and the current velocity profiles in the presence of either sinusoidal or nonlinear waves. Since the Longuet-Higgins-type streaming due to wave propagation is absent in OWT flows and not included in the semi-analytical model, future work is necessary to extend this study for applications in the coastal environment.

A direct numerical simulation study is conducted to investigate sinusoidal oscillatory flow over a two-dimensional wavy wall. The height and wavelength of the bottom profile, and the period and amplitude of the free-stream oscillation, are selected to mimic a wave-driven boundary layer over vortex ripples on a sandy seabed. Two cases with different Reynolds numbers $(Re)$ are considered, and the higher-$Re$ case achieves a fully developed turbulent state with a wide separation between the energy-containing and dissipative scales. The oscillatory flow is characterized by coherent columnar vortices, which are the main transport agents of turbulent kinetic energy and enstrophy. Two classes of coherent vortices are observed: (i) a primary vortex formed at the lee side of the ripple by flow separation at the crest; (ii) a secondary vortex formed beneath the primary vortex by vortex-induced separation. When the free-stream velocity weakens, these vortices form a counter-rotating vortex dipole and eject themselves over the crest with their mutual induction. Turbulence production peaks twice in a half-cycle; during the formation of the primary vortex and during the ejection of the vortex dipole. The intensity of the former peak remains low in the lower-$Re$ case, as the vortex dipole follows a higher altitude trajectory limiting its interactions with the bottom, and leaving minimal residual turbulence around the ripples for the subsequent half-cycle. Flow snapshots and spectral analysis reveal two dominant three-dimensional features: (i) an energetic vortex mode with a preferred spanwise wavelength close to the ripple wavelength; (ii) streamwise vortical structures in near-wall regions with a relatively shorter spanwise spacing influenced by viscous effects. The vortex mode becomes strong when the cores of the vortices are strained to an elliptical form while moving towards the crest. Following the detachment of the vortices from the ripple, the vortex mode in the higher-$Re$ case breaks down the spanwise coherence of the columnar vortices and decomposes them into intermittent patches of turbulent vortex clusters. The distribution of wall shear stress over the ripple is also analysed in detail. The peak values are observed near the ripple crest around the ejection of the vortex dipole and the maximum free-stream velocity. In the former, both the vortex mode and streamwise vortices have strong footprints on the wall, yielding a bimodal wall-shear-stress spectrum with two distinctive peaks. In the second high-stress regime, decaying coherent vortices impose strong inhomogeneity on the wall shear stress as their wall-attached parts sweep the ripples. These spanwise variations in the wall shear provide insights into the instability of two-dimensional sand ripples.

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Double staining flow cytometry was performed using 7-amino actinomycin D and 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate, to detect the level fluctuation of reactive oxygen species (ROS) during the cell cycle of normal NB4 cells. Our results showed that NB4 cells possessed higher level of ROS in G2/M phase than in G1 and S phases. Double staining flow cytometry, with TdT mediated dUTP nick end labeling (Tunel) and propidium iodide (PI), indicated that As 2 O 3 (2 µM) could induce apoptosis in NB4 cells prevailingly from G2/M phase, and this efficacy was enhanced upon co-administration of 2, 3-dimethoxy-1, 4-naphthoquinone (DMNQ) (2.5 µM) which could produce the endogenous ROS. These results suggested that different ROS level in different cell cycle phases of NB4 cells might determin the selective induction of G2/M apoptosis and the cells' susceptibility to apoptosis by As 2 O 3 .

The design of packed columns requires the detailed description of the hydrodynamics on the surface of the packings. To analyze the local flow behavior of the liquid phase, a three-dimensional Computational Fluid Dynamics (CFD) model was developed that applies to the two-phase countercurrent flow on an inclined and flat plate. This model, based on the volume-of-fluid (VOF) method, considers the gravity, the surface tension and the drag force between the two phases. The development of such a model allows investigation of the influences of the liquid and gas flow rates on the flow behavior such as the film flow and the rivulet flow. A validation of the model was performed using data from the literature and from experiments conducted in this work. Simulation and experimental results demonstrate that the specific wetted area on the plate decreases with decreasing liquid load. Moreover, CFD simulations reveal that the presence of the countercurrent gas phase tends to increase the fluctuation and the thickness of the film flow, which is in accordance with experimental data. It also affects the flow behavior of the rivulet flow and changes the velocity profiles for both film and rivulet flow behavior. On the other hand, the simulation results indicate that CFD is a potent tool for analyzing and investigating the flow phenomena in chemical engineering.

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