Raman spectroscopy and surface‐enhanced Raman spectroscopy (SERS) have been extensively explored in the design of accurate, transparent, and conclusive food safety and quality control assays. Its hyphenation with chemometric algorithms is instrumental in securing safe food campaigns. To provide valuable recommendations and meet the growing demands for food screening, the current study begins with a brief description of the Raman spectroscopy and SERS theory followed by a comprehensive overview of spectral preprocessing, qualitative algorithms, variable selection methods, and quantitative algorithms. The review emphasizes on the importance of food monitoring practices using multivariate regression models. The applicability of the distinct chemometrics modes toward monitoring pesticide, food and illicit additives, heavy metals, pathogens, and its metabolites in Raman spectroscopy and SERS is covered in dairy, poultry, oil, honey, beverages, and other selected food matrices. Its pertinence toward classification and/or discrimination in food quality and safety monitoring and authentication is examined. Finally, it also complies with the limitations, key challenges, and prospects. The chemometrics processing spectra implemented with simpler or no complicated sample pretreatment step make Raman spectroscopy/SERS technique a potential approach that is expected to achieve simultaneous and fast detection of multiple analytes in food matrices.
The
extensive use of 4-aminothiophenol (4-ATP) as a Raman-active
molecule is attributed to ease in its functionalization and use for
potential sensing in surface-enhanced Raman scattering (SERS) research.
However, its SERS spectrum is susceptible to be influenced and chemically
affected by several experimental factors. Thus, the current study
focuses on theoretically and experimentally investigating such physicochemical
factors and its counteractive stimulus to discriminate the SERS signal
arising from the analyte of interest and interferents. The sensing
mechanism of nitrite-triggered 4-ATP that might induce its oxidation
and/or dimerization and the conditions inhibiting or generating abnormal
type b2 bands (1142, 1388, and 1432 cm–1) were discussed. The factors covered were as follows: (i) solvent
effect; (ii) laser power; (iii) nature of the substrate; (iv) reaction
media; (v) reaction kinetics; and (vi) influence of air or any oxygen
(dissolved)-containing moiety. The activation of 4-ATP by any factor
forms 4,4-dimercaptoazobenzene with characteristic symmetric b2 bands in nearly all the literature, whereas, herein, the
species formed between nitrite and 4-ATP was differentiated and confirmed
by basic electronic assignments along with their respective binding
mechanism toward 1-naphthylamine. The retention mechanism of 4-ATP
was assigned and controlled at pH < 2.0, where the protonation
of amine and unavailability of necessary precursors in the presence
of excess H+ greatly resists its oxidation, making it independent
of many potential factors. Moreover, a highly stable platform for
nitrite sensing has been established with exceptional selectivity
(in the presence of potent interferences) and sensitivity (0.3 ×
10–8 M).
The current study assembles liquid-microextraction, surface enhanced Raman scattering (SERS) and chemometrics algorithms in one platform for chromium speciation using octahedral Cu2O@Ag nanocomposites (Cu2O@AgNCs) as a SERS substrate.
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