Randomly amplified polymorphic DNA analysis was conducted with 14 primers to 17 strains of Xylella fastidiosa. There was a high degree of similarity among the seven Pierce's disease (PD) strains (S xy > 0.93) and the seven oak leaf scorch (OLS) strains (S xy > 0.96). However, the two groups were different, with a similarity index of 0.67, confirming the presence of a PD DNA cluster and suggesting the presence of a new OLS cluster. The control plum leaf scald strains (two strains) together with the periwinkle wilt strain had a much smaller similarity index (0.44) compared with the PD and OLS clusters.
Pierce's disease (PD) strains of Xylella fastidiosa were identified by random amplified polymorphic DNA (RAPD) fingerprinting. Two random primers including OPA‐03 (agtcagccac) and OPA‐11 (caatcgccgt) were found to be efficient for differentiating PD strains isolated from a vineyard in North Florida in 1996 (129 strains) and 1997 (29 strains) from non‐PD strains of X. fastidiosa (citrus variegated chlorosis, mulberry leaf scorch, periwinkle wilt, plum leaf scald, and phony peach) and strains from Xanthomonas campestris pv. vesicatoria and Escherichia coli. This study shows that RAPD fingerprinting is a useful tool to supplement the conventional symptoms‐colony morphology‐slow growth identification procedure routinely used to identify the PD pathogen.
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