RAPD fingerprinting<i>Xylella fastidiosa</i>Pierce's disease strains isolated from a vineyard in North Florida

Albibi, R; CHEN, JING; Lamikanra, Olusola; Banks, Donna; Jarret, Robert L.; Smith, Barbara J.

doi:10.1111/j.1574-6968.1998.tb13168.x

Cited by 20 publications

(2 citation statements)

References 16 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Publications were filtered by selecting only records where the pathogen detection on symptomatic grapevines was confirmed by PCR or Elisa. The exact coordinates of the records were taken when available in the publication or approximated to locality or county level to build the Supplementary Data 1 19 , 39 , 41 , 75 – 79 . For modelling purposes and to attempt a general rough estimate of the R 0 parameter valid for the entire US, we assumed a single vector with a uniform spatial distribution.…”

Section: Methodsmentioning

confidence: 99%

Global predictions for the risk of establishment of Pierce’s disease of grapevines

Giménez-Romero

Galván

Montesinos³

et al. 2022

Commun Biol

View full text Add to dashboard Cite

The vector-borne bacterium Xylella fastidiosa is responsible for Pierce’s disease (PD), a lethal grapevine disease that originated in the Americas. The international plant trade is expanding the geographic range of this pathogen, posing a new threat to viticulture worldwide. To assess the potential incidence of PD, we have built a dynamic epidemiological model based on the response of 36 grapevine varieties to the pathogen in inoculation assays and on the vectors’ distribution when this information is available. Key temperature-driven epidemiological processes, such as PD symptom development and recovery, are mechanistically modelled. Integrating into the model high-resolution spatiotemporal climatic data from 1981 onward and different infectivity (R0) scenarios, we show how the main wine-producing areas thrive mostly in non-risk, transient, or epidemic-risk zones with potentially low growth rates in PD incidence. Epidemic-risk zones with moderate to high growth rates are currently marginal outside the US. However, a global expansion of epidemic-risk zones coupled with small increments in the disease growth rate is projected for 2050. Our study globally downscales the risk of PD establishment while highlighting the importance of considering climate variability, vector distribution, and an invasive criterion as factors to obtain better PD risk maps.

show abstract

Section: Methodsmentioning

confidence: 99%

Global predictions for the risk of establishment of Pierce’s disease of grapevines

Giménez-Romero

Galván

Montesinos³

et al. 2022

Commun Biol

View full text Add to dashboard Cite

show abstract

“…In the late eighties and early nineties, restriction fragment length polymorphism (RFLP) and randomly amplified polymorphic DNA (RAPD) analyses were largely diffused to study strain differentiations of pathogenic bacteria(Denny et al, 1988; Hartung and Civerolo, 1989; Grajalmartin et al, 1993; Cave et al, 1994; Albibi et al, 1998; da Costa et al, 2000; Ferreira et al, 2000; Mehta et al, 2000; Lacava et al, 2001; Su et al, 2008). In a study on X. fastidiosa , RFLP were used to characterize 24 strains of this pathogen from 8 different hosts (Chen et al, 1992).…”

Section: Hosts and Diseasesmentioning

confidence: 99%

Xylella fastidiosa: Host Range and Advance in Molecular Identification Techniques

Baldi

Porta

2017

Front. Plant Sci.

View full text Add to dashboard Cite

In the never ending struggle against plant pathogenic bacteria, a major goal is the early identification and classification of infecting microorganisms. Xylella fastidiosa, a Gram-negative bacterium belonging to the family Xanthmonadaceae, is no exception as this pathogen showed a broad range of vectors and host plants, many of which may carry the pathogen for a long time without showing any symptom. Till the last years, most of the diseases caused by X. fastidiosa have been reported from North and South America, but recently a widespread infection of olive quick decline syndrome caused by this fastidious pathogen appeared in Apulia (south-eastern Italy), and several cases of X. fastidiosa infection have been reported in other European Countries. At least five different subspecies of X. fastidiosa have been reported and classified: fastidiosa, multiplex, pauca, sandyi, and tashke. A sixth subspecies (morus) has been recently proposed. Therefore, it is vital to develop fast and reliable methods that allow the pathogen detection during the very early stages of infection, in order to prevent further spreading of this dangerous bacterium. To this purpose, the classical immunological methods such as ELISA and immunofluorescence are not always sensitive enough. However, PCR-based methods exploiting specific primers for the amplification of target regions of genomic DNA have been developed and are becoming a powerful tool for the detection and identification of many species of bacteria. The aim of this review is to illustrate the application of the most commonly used PCR approaches to X. fastidiosa study, ranging from classical PCR, to several PCR-based detection methods: random amplified polymorphic DNA (RAPD), quantitative real-time PCR (qRT-PCR), nested-PCR (N-PCR), immunocapture PCR (IC-PCR), short sequence repeats (SSRs, also called VNTR), single nucleotide polymorphisms (SNPs) and multilocus sequence typing (MLST). Amplification and sequence analysis of specific targets is also mentioned. The fast progresses achieved during the last years in the DNA-based classification of this pathogen are described and discussed and specific primers designed for the different methods are listed, in order to provide a concise and useful tool to all the researchers working in the field.

show abstract

Xylella

Saddler¹,

Bradbury²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

Xy.lel' la . Gr. n. xylon wood; M.L. dim. ending ‐ ella; M.L. fem. n. Xylella a small wood. Proteobacteria / Gammaproteobacteria / Xanthomonadales / Xanthomonadaceae / Xylella Single, straight rods, 0.25–0.35 × 0.9–3.5 μm , that can form long filamentous strands under some cultural conditions. Gram negative. Nonmotile , lacking flagella. Endospores not produced. Aerobic , having a strictly respiratory type of metabolism with oxygen as the terminal electron acceptor. Colonies are cream to white . Two colonial variants are frequently encountered: convex to pulvinate, smooth, opalescent with entire margins; and umbonate, rough with finely undulated margins. Oxidase negative and catalase positive . Optimum temperature for growth, 26–28°C; optimum pH for growth, 6.5–6.9. Nutritionally fastidious. Found mainly in the xylem of plant tissue. The mol % G + C of the DNA is : 51.0–52.4 ( T m ); 52–53.1 (Bd). Type species : Xylella fastidiosa Wells, Raju, Hung, Weisburg, Mandelco‐Paul and Brenner 1987, 141.

show abstract

RAPD fingerprintingXylella fastidiosaPierce's disease strains isolated from a vineyard in North Florida

Cited by 20 publications

References 16 publications

Global predictions for the risk of establishment of Pierce’s disease of grapevines

Global predictions for the risk of establishment of Pierce’s disease of grapevines

Xylella fastidiosa: Host Range and Advance in Molecular Identification Techniques

Xylella

Contact Info

Product

Resources

About