A bi-enzyme PEC biosensor was explored for the sensitive determination of phosphatidylcholine (PC) in vegetable oil based on phospholipase D (PLD) and choline oxidase (ChOx) immobilized on an indium tin oxide (ITO) electrode. The bi-enzyme electrode is based on modifying SnO2 nanoparticles (SnO2 NPs) and polythionine (PTh). SnO2 NPs were obtained by hydrothermal method. The electropolymerization was performed to load the PTh layer on the ITO/SnO2 NPs electrode surface. The immobilization method of PLD and ChOx combined embedding and cross-linking. This bi-enzyme PEC biosensor with ITO/SnO2 NPs/PTh/ChOx/PLD electrode was developed for PC detection. Under the optimized conditions, a good linear relationship was obtained in the range of 0.01 mM to 5 mM with a detection limit of 0.002 (S/N=3). The proposed PEC analysis method shows good reproducibility, selectivity, and stability. The prepared PEC biosensor was used to detect PC in vegetable oil, and the results were consistent with those of liquid chromatography. This is an effective PEC sensing strategy for the rapid and sensitive determination of PC in vegetable oil.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.