A tailor-made colorimetric and red-emitting fluorescent dual probe for G-quadruplex nucleic acids was developed by incorporating a coumarin-hemicyanine fluorophore into an isaindigotone framework. The significant and distinct changes in both the color and fluorescence of this probe enable the label-free and visual detection of G-quadruplex structures.
Transcriptional control of c-myc oncogene is an important strategy for antitumor drug design. G-quadruplexes in the promoter region have been proven to be the transcriptional down-regulator of this gene. The transcriptional factor NM23-H2 can reactivate c-myc transcription by unwinding the G-quadruplex structure. Thus, down-regulation of c-myc transcription via disrupting G-quadruplex-NM23-H2 interaction might be a potential approach for cancer therapy. Here, a series of new isaindigotone derivatives were designed and synthesized based on our previous study. The abilities of these derivatives on interacting with G-quadruplexes or NM23-H2, and disrupting G-quadruplex-NM23-H2 interaction were evaluated. Among these derivatives, 19d and 22d showed remarkable abilities on disrupting G-quadruplex-NM23-H2 interaction. They exhibited significant effects on c-myc-relating processes in SiHa cells, including inhibiting the transcription and translation, inhibiting cellular proliferation, inducing apoptosis, and regulating cell cycle. Our findings provided the basis for the anticancer strategy based on c-myc transcriptional regulation via small molecules disrupting G-quadruplex-protein interaction.
Two novel meso-CF3 BODIPY-based fluorescent rotors have been rationally prepared and sensitively respond to viscosity in living cells with fluorescence “turn-on” effect, attributing to the special restricted rotation of meso-CF3...
The rapid and convenient method for identification of all kinds of G-quadruplex is highly desirable. In the present study, a novel colorimetric indicator for a vast variety of G-quadruplex was designed and synthesized on the basis of thiazole orange and isaindigotone skeleton. Its distinct color change enables label-free visual detection of G-quadruplexes, which is due to the disassembly of dye H-aggregates to monomers. This specific detection of G-quadruplex arises from its end-stacking interaction with G-quartet. On the basis of this universal indicator, a facile approach for large-scale identification of G-quadruplex was developed.
Meso-substituted
boron dipyrromethenes (BODIPYs) provide a potential
and innovative strategy for the synergistic construction of aggregation-induced
emission (AIE) probes and fluorescent rotors for monitoring cellular
viscosity changes, which play critical roles in understanding the
function of viscosity in its closely associated diseases. Therefore,
for the first time, a BODIPY-based fluorescent probe (1) with a rotatable meso-benzothiazole group was
rationally designed and synthesized, showing both good viscosity-responsive
and AIE properties. Probe 1 through direct linkage with
the thiazole group, showed nearly no emission in low viscous solvents;
however, a strong emission at 534 nm appeared and increased gradually
with the increase in viscosity, attributing to the efficient restriction
of the rotatable meso-benzothiazole group. The intensity
(log I
534) displayed a good linear relationship
with viscosity (log η) in the viscous range of 0.59–945
cP in methanol/glycerol mixtures. Interestingly, 1 showed
enhanced emission at 534 nm in 70% water compared to pure acetonitrile
due to the aggregation-induced inhibited rotations. Cellular imaging
suggested that 1 could successfully sense lysosomal viscosity
changes induced by lipopolysaccharide, nystatin, low temperature,
and dexamethasone in living cells, which could be further applied
in autophagy monitoring by tracing viscosity changes. As a comparison,
its analogue 2 directly linking with the phenyl group
showed no viscosity-responsive or AIE properties. Therefore, for the
first time, we reported a meso-benzothiazole-BODIPY-based
fluorescent rotor with AIE and lysosomal viscosity-responsive properties
in nervous cells, which was further applied in monitoring autophagy,
and this work thus could provide an innovative strategy for the design
of potential AIE and viscosity-responsive probes.
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