In living organisms, changes in calcium flux are integral to many different cellular functions and are especially critical for the activity of neurons and myocytes. Genetically encoded calcium indicators (GECIs) have been popular tools for reporting changes in calcium levels in vivo. In particular, GCaMPs, derived from GFP, are the most widely used GECIs and have become an invaluable toolkit for neurophysiological studies. Recently, new variants of GCaMP, which offer a greater variety of temporal dynamics and improved brightness, have been developed. However, these variants are not readily available to the Caenorhabditis elegans research community. This work reports a set of GCaMP6 and jGCaMP7 reporters optimized for C. elegans studies. Our toolkit provides reporters with improved dynamic range, varied kinetics, and targeted subcellular localizations. Besides optimized routine uses, this set of reporters is also well-suited for studies requiring fast imaging speeds and low magnification or low-cost platforms.

Background: An increasing number of patients with chronic illnesses have implanted cardiac rhythm devices such as pacemakers and implantable cardioverter-defibrillators (ICDs). This study was conducted to identify potentially useful predictors of in-hospital cardiac arrest (I-HCA) within paced electrocardiogram (ECG) signals from cardiovascular patients with implanted medical devices. Methods:In this retrospective study of 17 subjects, full-disclosure ECG traces prior to the time of documented I-HCA were analyzed to determine R-R intervals and QRS durations (QRSd).Results: Ventricular paced QRSd prolongation was observed prior to I-HCA in 10/16 (63%) subjects. QRSd was significantly greater immediately preceding cardiac arrest than during each of the 8 hours prior to cardiac arrest (P < 0.05). Heart rate changes (measured using standard deviation) within 15 minutes of cardiac arrest were significantly greater in subjects with pulseless electrical activity (PEA)/asystolic arrest compared to those with cardiac arrests due to ventricular tachycardia/ventricular fibrillation (VT/VF) (10.13 vs 3.31; P = 0.024). Significant differences over the 8 hours preceding cardiac arrest in heart rate (74 vs 86 beats/min; P = 0.002) and QRS duration (172 ms vs 137 ms; P < 0.001) were observed between subjects with initial rhythms of VT/VF and those with initial rhythms of PEA/asystole.

In living organisms, changes in calcium flux are integral to many different cellular functions and are especially critical for the activity of neurons and myocytes. Genetically encoded calcium indicators (GECIs) have been popular tools for reporting changes in calcium levelsin vivo. In particular, GCaMP, derived from GFP, are the most widely used GECIs and have become an invaluable toolkit for neurophysiological studies. Recently, new variants of GCaMP, which offer a greater variety of temporal dynamics and improved brightness, have been developed. However, these variants are not readily available to theCaenorhabditis elegansresearch community. This work reports a set of GCaMP6 and jGCaMP7 reporters optimized forC. elegansstudies. Our toolkit provides reporters with improved dynamic range, varied kinetics, and targeted subcellular localizations. Besides optimized routine uses, this set of reporters are also well-suited for studies requiring fast imaging speeds and low magnification or low-cost platforms.

This paper proposes a passive multi‐view system for human body shape reconstruction, namely RHF‐Human, to overcome several challenges including accurate calibration and stereo matching in self‐occluded and low‐texture skin regions. The reconstruction process includes four steps: capture, multi‐view camera calibration, dense reconstruction, and meshing. The capture system, which consists of 90 digital single‐lens reflex cameras, is single‐shot to avoid nonrigid deformation of the human body. Two technical contributions are made: (1) a two‐step robust multi‐view calibration approach that improves calibration accuracy and saves calibration time for each new human body acquired and (2) an accurate PatchMatch multi‐view stereo method for dense reconstruction to perform correct matching in self‐occluded and low‐texture skin regions and to reduce the noise caused by body hair. Experiments on models of various genders, poses, and skin with different amounts of body hair show the robustness of the proposed system. A high‐fidelity human body shape dataset with 227 models is constructed, and the average accuracy is within 1.5 mm. The system provides a new scheme for the accurate reconstruction of nonrigid human models based on passive vision and has good potential in fashion design and health care.