northern blotting and (4) comparative genomic analysis with distant vertebrate species such as Fugu rubripes and Tetraodon nigroviridis. This transcript-mapping strategy has identified 32 potential transcription units, including 2 known genes, 5 new genes, 9 Unigene entries and 16 other expressed sequence tag clusters. The region also contains five pseudogenes. The map should facilitate subsequent efforts to characterize the candidate genes. This study illustrates how the integration of genome-based approaches facilitates the identification of genes in a large interval.
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