This study was conducted to investigate low-cost substrate alternative and the optimum culture conditions in mass producing the local marine thraustochytrid, Schizochytrium sp. LEY7 isolated from the mangrove leaves of Baybay, Southern Leyte Philippines. Results showed that Schizochytrium sp. LEY7 is able to utilize commercial grade glucose and yeast extract from NaCl-treated baker's yeast as source of carbon and micronutrients respectively. Cost of mass producing the thraustochytrid isolate using the alternative production substrates was substantially reduced. Incubation temperature and salinity levels were the two growth factors significantly affecting the biomass production of the isolate. The short duration of lag phase shown by the isolate suggests a growth advantage in that cells are readily able to adapt to their new environment. Total lipids averaged to 19.4%. Principal fatty acids were palmitic acid (C16:0) with 33.52% and docosahexanoic acid (DHA, C22:6n-3) with 39.92% proportion to total fatty acid. Eicosapentaenoic (C20:5n-3, 1.01%) and arachidonic (C20:4n-3, 0.90%) are present but in relatively lower amount. Our findings showed the potential of low-cost substrate in mass producing the local thraustochytrid isolate, Schizochytrium sp. LEY7 as lipid and polyunsaturated fatty acid source in aquaculture. Biomass production was enhanced by optimizing the culture conditions.
Numerous studies have been conducted on ballast water species composition and diversity in other countries but not in the Philippines. Thus, this study aimed to provide baseline information on the plankton diversity in ballast water of the inter-island passenger-cargo vessel calling the ports of Iloilo-Bacolod-Manila-Cagayan de Oro, Philippines and vice-versa. Specifically, this study aimed to determine the presence of phytoplankton and zooplankton diversity and species density of this plankton measured in cells/ml using the haemacytometer technique. Composite sampling was employed having one liter of ballast water used per ballast tank. A total number of 15 genera of phytoplankton (diatom) and one genus of zooplankton were recorded. Chroococcus, Nannochloris and Protococcus had the highest cells/ml while Ankistrodesmus, Micromonas and Synedra had the lowest cells/ml. The most common phytoplankton observed in ballast tanks were Nannochloris and Protococcus. Neocalanus (copepod) was present in all ballast tanks except in ballast tank 1 (fore-peak). The phytoplankton and zooplankton composition was found to be non-invasive in nature showing its ubiquity in the marine environment. This study provides an initial assessment or preliminary list of phytoplankton and zooplankton diversity from the ballast water of a passenger-cargo vessel calling the Philippine ports.
Diverse array of microorganisms such as bacteria, fungi and protists are involved during oil spill. Each microorganism has its own specific function whether it has to degrade or adsorb hydrocarbons. One important microorganism is the Thraustochytrid that is a fungoid protist and are common in marine and estuarine habitats. Numerous studies existed on the biodegradation and adsorption of Thraustochytrids on various substances but not on Bunker C oil. Thus, this study aimed to determine the adsorption capacity and mean biomass of Thraustochytrids in Bunker C oil using different cell densities measured in grams. All of the three treatments or cell densities (1 x 105 cells ml-1 , 1 x 106 cells ml-1 and 1 x 107 cells ml-1) were triplicated and average values were recorded. Oil dispersant was used as a control. It showed that Thraustochytrid with 1 x 107 cells ml-1 showed the highest adsorbed oil (.057 ḡ) among the three cell densities and showed significant difference at p = .01 but comparable to the control (.066 ḡ). In terms of biomass concentration, all cell densities showed no significant difference at p = .01. Thraustochytrid is a promising tool during oil spill because it has the capacity to adsorb oil.
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