Biomass production, proximate composition and fatty acid profile of the local marine thraustochytrid isolate,Schizochytriumsp. LEY7 using low-cost substrates at optimum culture conditions

Abstract

This study was conducted to investigate low-cost substrate alternative and the optimum culture conditions in mass producing the local marine thraustochytrid, Schizochytrium sp. LEY7 isolated from the mangrove leaves of Baybay, Southern Leyte Philippines. Results showed that Schizochytrium sp. LEY7 is able to utilize commercial grade glucose and yeast extract from NaCl-treated baker's yeast as source of carbon and micronutrients respectively. Cost of mass producing the thraustochytrid isolate using the alternative production substrates was substantially reduced. Incubation temperature and salinity levels were the two growth factors significantly affecting the biomass production of the isolate. The short duration of lag phase shown by the isolate suggests a growth advantage in that cells are readily able to adapt to their new environment. Total lipids averaged to 19.4%. Principal fatty acids were palmitic acid (C16:0) with 33.52% and docosahexanoic acid (DHA, C22:6n-3) with 39.92% proportion to total fatty acid. Eicosapentaenoic (C20:5n-3, 1.01%) and arachidonic (C20:4n-3, 0.90%) are present but in relatively lower amount. Our findings showed the potential of low-cost substrate in mass producing the local thraustochytrid isolate, Schizochytrium sp. LEY7 as lipid and polyunsaturated fatty acid source in aquaculture. Biomass production was enhanced by optimizing the culture conditions.

Evaluation of growth and fatty acid profile of Nile tilapia (Oreochromis niloticus) fed with Schizochytrium sp.

“…provided the same profile of fatty acids, however in different amounts, mainly from the omega‐3 family. Ludevese‐Pascual Dela Peña and Tornalejo (), stand out that the fresh Schizochytrium sp. has an average weight of 19.4%‐total lipids.…”

Effect of pH on Schizochytrium limacinum Production Grown Using Crude Glycerol and Biogas Digestate Effluent

“…The nutritional value of the different species which belong to the family of Thraustocytrids is species-specific and differs with respect to culture conditions and culture media composition [46]. According to our results, at laboratory conditions, the total protein content exhibited an elevated fraction at high pH levels with reduced biomass productivity.…”

Occurrence of Thraustochytrids on fallen mangrove leaves from Pagbilao Mangrove Park, Quezon Province

“…(Aki et al, 2003;Furlan et al, 2017;Huang et al, 2001;Singh et al, 2016;Ugalde et al, 2018;Yamasaki et al, 2006). Despite the rich mangrove habitats in the Philippines, there are but a handful of researches conducted here (Arafiles et al, 2011;Atienza et al, 2012;Dela Peña et al, 2016;Estudillo-Castillo et al, 2009;Leaño, 2001;Leaño et al, 2003;Ludevese -Pascual et al, 2016;Oclarit & Hepowit, 2007;Uba et al, 2016). This research further investigates, supplements additional information, and adds to the number of researches on thraustochytrids in the country.…”