Wetland degradation makes significant impacts on soil, and bacterial communities in soil are likely to respond to these impacts. The purpose of this study was to investigate the impacts of soil property, soil type and soil depth on bacterial community in different stages of soil degradation in the Zoige Wetland. Microbial biomass carbon was estimated from chloroform fumigation-extraction. Bacterial communities were evaluated by cluster and principal component analysis of DGGE banding patterns and sequencing of partial 16S rDNA PCR amplicons. Experimental results showed that microbial biomass carbon decreased with the soil types (Peat soil > Swamp soil > Meadow soil > Sandy soil) and declined with soil depths (0-20 > 20-40 > 40-60 cm). Bacterial community was affected by soil type more primarily than by soil depth. In addition, the microbial biomass carbon was strongly correlated with soil water content, soil organic carbon and total nitrogen. Sequence analysis of DGGE bands indicated that bacterial phyla of α-Proteobacteria, γ-Proteobacteria, Bacteroidetes, Flavobacterium and Unidentified bacterium predominantly existed in the soil. All these results suggest that specific changes in soil property, soil type and soil depth affected soil bacterial community both quantitatively and qualitatively.

The purpose of this study was to analyze the feasibility and accuracy of a newly developed guide apparatus for the percutaneous retrograde lag screw fixation of posterior column of acetabular fractures. 3D pelvic models were reconstructed from the helical computed tomographic data of 33 adult patients using the Mimics 10.01 software. The virtual cylindrical implants were placed along a line passing through the central point of the ischial tuberosity and the midpoint between the most prominent point of anterior superior iliac spine and that of posterior superior iliac spine. Some anatomical parameters were then measured, based on which a guide apparatus was developed, and its safety and accuracy were experimentally validated with pelvic and cadaveric specimens. The screws were successfully placed in all of the 66 hemipelves. There was a significant difference between the male and female groups in the AB distance (156.26 ± 7.28 mm and 151.38 ± 8.11 mm), OI distance (139.53 ± 7.56 mm and 125.15 ± 11.17 mm), and diameter (12.19 ± 1.97 mm and 10.19 ± 2.14 mm) of the virtual cylindrical implants. This guide apparatus was proved effective for percutaneous retrograde lag screw fixation of posterior column acetabular fractures by the experiments with the pelvic and cadaveric specimens. Screw fixation of posterior column fractures via OI is safe and feasible. We designed a new percutaneous retrograde screw fixation guide apparatus to assist internal fixation of posterior column acetabular fracture.

Kinetic roughening of tantalum films during the initial growth stages has been studied by atomic force microscopy, scanning electron microscopy, and dynamic scaling theory. Different from the time-independent scaling behavior for continuous film growth, an intriguing unstable kinetic roughening occurs during island coalescence. In such case, roughness exponent α increases with growth time, accompanied by lower growth exponent β and higher coarsening exponent η. Detailed analysis of film surface morphology and simple phenomenological models suggests that this unstable behavior is related to the pronounced lateral growth of surface islands, which arises from the combined effect of the formation of grain boundary and the covering of heterogeneous substrate surface.

Molecular docking is a structure-based and computer-aided drug design approach that plays a pivotal role in drug discovery and pharmaceutical research. AutoDock is the most widely used molecular docking tool for study of protein–ligand interactions and virtual screening. Although many tools have been developed to streamline and automate the AutoDock docking pipeline, some of them still use outdated graphical user interfaces and have not been updated for a long time. Meanwhile, some of them lack cross-platform compatibility and evaluation metrics for screening lead compound candidates. To overcome these limitations, we have developed Dockey, a flexible and intuitive graphical interface tool with seamless integration of several useful tools, which implements a complete docking pipeline covering molecular sanitization, molecular preparation, paralleled docking execution, interaction detection and conformation visualization. Specifically, Dockey can detect the non-covalent interactions between small molecules and proteins and perform cross-docking between multiple receptors and ligands. It has the capacity to automatically dock thousands of ligands to multiple receptors and analyze the corresponding docking results in parallel. All the generated data will be kept in a project file that can be shared between any systems and computers with the pre-installation of Dockey. We anticipate that these unique characteristics will make it attractive for researchers to conduct large-scale molecular docking without complicated operations, particularly for beginners. Dockey is implemented in Python and freely available at https://github.com/lmdu/dockey.

This study suggest that both kinds of vein grafts play positive roles in facial nerve regeneration after being repaired immediately, but the autogenous inside-out vein grafts might accelerate and facilitate axonal regeneration as compared with control.

No abstract

The effects of the additives rich in aromatics on the intensification of vacuum distillation were investigated with the viscosity measurement, a simulated distillation analyzer, a Kjeldahl vacuum distillation unit and a True Boiling Point distillation unit. The temperature of liquid phase could influence the effects of additives. The hot-stage microscope was adopted to study qualitatively the intensification mechanism of the additives. When the temperature was lower than 320'C, it was found that the additives could help the lighter fractions going out which had been adsorbed by the core and constituted the outer layers of the colloidal particles. Nevertheless, when the temperature was high (above 380'C), the colloidal particles in the atmospheric residue almost disappeared and the distillation yield could not be increased obviously by the additives.

A magnetically confined ambient ion source enables a great improvement in the MS signal intensity and detection sensitivity.