RNA modifications, including RNA methylation, are widely existed in cutaneous melanoma (CM). Among epigenetic modifications, N7-methylguanosine (m7G) is a kind of modification at 5' cap of RNA which participate in maintaining the stability of mRNA and various cell biological processes. However, there is still no study concerning the relationship between CM and m7G methylation complexes, METTL1 and WDR4. Here, long non-coding RNA (lncRNAs) and gene expression data of CM from the Cancer Genome Atlas (TCGA) database were retrieved to identify differentially expressed m7G-related lncRNAs connected with overall survival of CM. Then, Cox regression analyses was applied to construct a lncRNA risk signature, the prognostic value of identified signature was further evaluated. As a result, 6 m7G-associated lncRNAs that were significantly related to CM prognosis were incorporated into our prognostic signature. The functional analyses indicated that the prognostic model was correlated with patient survival, cancer metastasis, and growth. Meanwhile, its diagnostic accuracy was better than conventional clinicopathological characteristics. The pathway enrichment analysis showed that the risk model was enriched in several immunity-associated pathways. Moreover, the signature model was significantly connected with the immune subtypes, infiltration of immune cells, immune microenvironment, as well as several m6A-related genes and tumor stem cells. Finally, a nomogram based on the calculated risk score was established. Overall, a risk signature based on 6 m7G-associated lncRNAs was generated which presented predictive value for the prognosis of CM patients and can be further used in the development of novel therapeutic strategies for CM.
Chitosan modified by γ-ray-induced grafting polymerization of tributyl-(4-vinylbenzyl)phosphonium chloride can be used as a safe and high-efficiency gene carrier.
Biosafe nanoparticles with strong near‐infrared (NIR) light photothermal conversion effect can bring effective hyperthermia as one of the promising approaches in cancer therapy. In this work, a new facile and green preparation method of polypyrrole (PPy) nanoparticles based on 60Co γ‐ray radiation on a simple air‐saturated strong acidic aqueous solution of pyrrole (pH ≤ 1) is studied. According to the MCAP‐FACSIMILE simulation on the concentrations of the radiolysis products of water at the presence of H+ and O2, the main strong oxidative radiolysis products ·OH and H2O2 rapidly induce the polymerization of pyrrole. The size of the prepared PPy nanoparticles is about several tens of nanometers and can be controlled by the pH, the concentration of the stabilizer poly(vinyl alcohol), and the absorbed dose rate (the amount of energy absorbed per unit mass of the irradiated material within per unit of time). The PPy nanoparticles show rapid and remarkable NIR (808 nm) photothermal conversion efficiency up to 40.1% in water. Furthermore, the in vitro and in vivo experiments confirm that the prepared PPy nanoparticles exhibit enough strong NIR photothermal effect in tumor cells (4T1 and HeLa) and show a promising prospect as the NIR photothermal agent for the future cancer therapy.
pEGFP-loaded thiolated and N-alkylated chitosan particles coated by a PEGylated hydroxybutyl chitosan shell show a good sustainable gene transfection effect.
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