Broussonetia papyrifera (BP) is a woody roughage source with high protein content. The experiment was conducted to explore the effects of different growth height (GH) on the BP yield, chemical composition, silage fermentation profile, as well as ruminants in vitro and in situ digestibility of different parts of BP. The three different harvested GH of BP were 0.8, 1.2, and 1.6 m, respectively. Samples from leaf, stem, and whole plant of BP were collected (each one has three replicates), making silage, and detected the nutritional composition of them. The results were analyzed by one-way analysis of variance with Duncan’s multiple comparisons. Fresh weight increased with the GH increased (P < 0.05). No significant difference was observed in dry matter (DM) and crude protein (CP) yield of leaf, and CP yield of whole plant between 1.2 to 1.6 m GH (P > 0.05). With the increase of GH, neutral detergent fiber (NDF) of BP increased, while CP content decreased (P < 0.05). Stem had the highest NDF and acid detergent fiber (ADF) content, and the lowest CP content and buffer capacity. The BP silage fermentation quality was deteriorated (lactic acid content decreased and pH values increased) with GH increased. For the different parts of BP, leaf silage had the highest pH and stem silage had the highest lactic acid content (P < 0.05). The leaf and its silage had the highest in vitro dry matter digestibility and gas production compared to others. The BP in situ digestibility were corresponded with in vitro results. DM, CP, NDF, and ADF effective digestibility rates of whole plant with 1.2 m GH were 439.6, 455.1, 412.9, and 381.3 g/kg, respectively. In conclusion, the BP nutritional quality decreased with GH increased and it could be used as a potential feedstuff for ruminants.
A two way factorial experiment was designed to study the technique aspects of ensiling rice straw (RS) mixed with Strawzyme (an experimental preparation of cell-wall degrading enzymes) and wheat bran (WB). The WB was added at levels of 0, 3, 6 or 9% (fresh basis), respectively, and the RS was untreated (C-0, C-3, C-6 and C-9), or treated with Strawzyme at level of 1300 g/t DM (T-0, T-3, T-6 and T-9). Addition of WB improved the fermentation quality and nutritive value of RS silage. The pH value, percentage of ammonia N in total N and butyrate content were decreased, and lactate content and in vitro gas production (GP) increased with the increasing levels of WB. Enzyme treatment decreased the neutral detergent fibre, pH, ammonia-N and butyrate, and increased lactate and in vitro GP. There were significant interaction effects on pH, lactate and butyrate content between enzyme and WB. Therefore the silages in T-6 and T-9 showed good quality with lowest pH and highest lactate as well as no any butyrate. Proportion of ammonia N of total N was decreased by 37.9 or 15.5%, and the lactic acid was increased by 67.8 or 5.7%, respectively, when Strawzyme plus 6 or 9% WB was added. It can be concluded that combination of Strawzyme with WB was more effective in the improvement of RS silage quality than addition of WB alone.
Serpin family A member 1 (SERPINA1) encodes a protease inhibitor participating in many human diseases, but its value in immunoregulation and prognosis of human cancers remains unclear. In this study, through comprehensive analysis of data from The Cancer Genome Atlas (TCGA) datasets, we found that SERPINA1 was dysregulated in many cancers compared with normal tissues. SERPINA1 expression was significantly associated with prognosis, immune subtype, molecular subtype, immune checkpoint (ICP) genes, tumor mutational burden (TMB), microsatellite instability (MSI), and the estimation of stromal and immune cells in malignant tumor tissues using expression data (ESTIMATE) score. There was a strong connection between SERPINA1 expression and tumor-infiltrating lymphocytes, and SERPINA1 showed significant relation to gene markers of immune cells in digestive tumors. Fluorescence-based multiplex immunohistochemistry confirmed that SERPINA1 protein expression was related to clinicopathologic features and immune infiltrates in hepatic cancer. This study suggests that SERPINA can potentially serve as a novel biomarker for cancer prognosis and immunotherapy.
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