Actin is a multi-functional gene family that can be divided into muscle-type actins and non-muscle-type actins. In this study, 37 unigenes encoding actins were identified from RNA-Seq data of Pacific white shrimp, Litopenaeus vannamei. According to phylogenetic analysis, four and three cDNAs belong to cytoplasmic- and heart-type actins and were named LvActinCT and LvActinHT, respectively. 10 cDNAs belong to the slow-type skeletal muscle actins, and 18 belong to the fast-type skeletal muscle actins; they were designated LvActinSSK and LvActinFSK, respectively. Some muscle actin genes formed gene clusters in the genome. Multiple alternative transcription starts sites (ATSSs) were found for LvActinCT1. Based on the early developmental expression profile, almost all LvActins were highly expressed between the early limb bud and post-larval stages. Using LvActinSSK5 as probes, slow-type muscle was localized in pleopod muscle and superficial ventral muscle. We also found three actin genes that were down-regulated in the hemocytes of white spot syndrome virus (WSSV)- and Vibrio parahaemolyticus-infected L. vannamei. This study provides valuable information on the actin gene structure of shrimp, furthers our understanding of the shrimp muscle system and helps us develop strategies for disease control and sustainable shrimp farming.
The main purpose of this study was to analyze the effect of coal mining on the springs in the Yushenfu mining area of China. The results of two springs and hydrological surveys conducted in 1994 and 2015 were compared to study the occurrence and evolution of springs before and after large-scale mining. The mechanism of spring evolution and ecological effects of domain evolution were analyzed by combined groundwater monitoring and evaluation of coal mining intensity. The results show that the maximum amount of single water inflow of spring with sand infiltration recharge was more than 10 L/s, the total amount of single water inflow of spring with mixed infiltration recharge was the highest, and the ecological effect of spring with loess infiltration recharge was the most significant. In the study area, 2580 springs (group) were distributed with a total flow of 4998.9428 L/s before 1994 and 376 residual springs (group) were present with a total flow of 996.392 L/s in 2015. Large-scale mining decreased the regional groundwater level, thus decreasing the amount of spring water. The high intensity of mining decreased the number of springs (group) and area of water and wetland in the study area. This directly affected the watershed ecology; the ecological degradation was significant.
In this study, a total number of 1,026 Chinese adolescents were surveyed using the cohesion sub-scale of the Family Environment Scale, the Self-control Scale, the Parental Monitoring Questionnaire, and the revised Olweus Bully/Victim Questionnaire to explore the effects of family cohesion on adolescents’ engagement in school bullying and the mechanisms of self-control and parental monitoring in the relationship between them. The results showed that: (1) family cohesion, self-control, and parental monitoring were significantly and negatively related to school bullying; (2) family cohesion directly influenced school bullying and also indirectly influenced school bullying through a mediating effect – self-control; (3) parental monitoring played a moderating role in the path of self-control affecting school bullying. Therefore, to reduce the occurrence of school bullying, it is necessary to strengthen the self-control ability of adolescents and improve the family cohesion environment and maintain a moderate level of parental monitoring. The results of this study revealed the effect of family cohesion on adolescents’ engagement in school bullying and its mechanism of action, which can provide a theoretical basis for preventing and reducing the occurrence of school bullying incidents.
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