Wnt gene family members and their expression profiling in Litopenaeus vannamei

Du, Jiangli; Zhang, Xiaojun; Yuan, Jianbo; Zhang, Xiaoxi; Li, Fuhua; Xiang, Jianhai

doi:10.1016/j.fsi.2018.03.034

Cited by 37 publications

(26 citation statements)

References 35 publications

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“…According to our investigation, the situation in the silkworm seems to be different. We detected a small amount of expression of BmWnt5 and BmWnt9 ; however, Wnt11 and WntA were highly expressed in silkworm nerves (Figure 7), which is consistent with the study on Litopenaeus vannamei [47], suggesting that the two genes may have functions in the nerves of Ecdysozoa.…”

Section: Discussionsupporting

confidence: 90%

Genome-Wide Identification and Expression Profiling of Wnt Family Genes in the Silkworm, Bombyx mori

Ding

Liu

Zheng

et al. 2019

IJMS

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Wnt is a family of conserved glycoproteins that participate in a variety of important biological processes including embryo development, cell proliferation and differentiation, and tissue regeneration. The Wnt family is a metazoan novelty found in all animal phyla. Studies have revealed that the number of Wnt genes varies among species, presumably due to reproduction and loss of genes during evolution. However, a comprehensive inventory of Wnt genes in Lepidoptera is lacking. In this study, we identified the repertoire of Wnt genes in the silkworm and seven other species of Lepidoptera and obtained eight Wnt genes (Wnt1, Wnt5–Wnt7, Wnt9–Wnt11, and WntA) in each species. Four of these Wnt genes are clustered in two orientations (5′-Wnt9-Wnt1-Wnt6-Wnt10-3′ and 5′-Wnt10-Wnt6-Wnt1-Wnt9-3′) in both moths and butterflies. Transcript analysis of Wnt in silkworm embryonic stages showed that each BmWnt gene had a unique expression pattern during embryological development. Analysis of a larval stage revealed differential expression of Wnt family members in diverse tissues. Our study provides an overview of the Wnt family in Lepidoptera and will inspire further functional study of the Wnt genes in the silkworm.

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Section: Discussionsupporting

confidence: 90%

Genome-Wide Identification and Expression Profiling of Wnt Family Genes in the Silkworm, Bombyx mori

Ding

Liu

Zheng

et al. 2019

IJMS

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“…There is evidence that in mammals, the Wnt/β-catenin pathway may be modulated by CRH, and Wnt mRNA abundance is clearly upregulated by ACTH (Khattak et al, 2010;Kuulasmaa et al, 2008). The Wnts are a family of secreted glycoproteins that include identified 19 Wnt ligands in mammals and 12 Wnt ligands in L. vannamei (Logan and Nusse, 2004;Du et al 2018). The canonical Wnt/β-catenin pathway is centered on regulating the levels of its major effector, β-catenin.…”

Section: Discussion Neuroendocrine and Signal Transduction Responses mentioning

confidence: 99%

Ammonia-N exposure alters neurohormone levels in the hemolymph and mRNA abundance of neurohormone receptors and associated downstream factors in the gills ofLitopenaeus vannamei

Pan

Wang

et al. 2019

Journal of Experimental Biology

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Effects of ammonia-N (0.05, 2, 10 and 20 mg l −1 ) on the neuroendocrine regulation of ammonia transport were investigated in Litopenaeus vannamei. The results showed that corticotrophinreleasing hormone, adrenocorticotropic hormone, dopamine, noradrenaline and 5-hydroxytryptamine concentrations in all ammonia-N groups increased significantly between 3 and 12 h. Cortisol increased significantly between 3 and 24 h. All hormones except crustacean hyperglycemic hormone were reduced to control levels. mRNA abundance of guanylyl cyclase increased significantly during the experiment. Dopamine receptor D 4 and α 2 adrenergic receptor mRNA abundance in treatments decreased significantly at the beginning, and eventually returned to the control level, whereas mRNA abundance of the 5-HT 7 receptor increased significantly only within the first 12 h. Changes in protein kinase (PKA, PKG) mRNA abundance were similar to the patterns of biogenic amines and crustacean hyperglycemic hormone, peaking at 6 and 12 h, respectively, whereas PKC mRNA abundance decreased within 24 h. 14-3-3 protein, FXYD2 and cAMP-response element binding protein mRNA abundance increased significantly and peaked at 6 h. β-catenin and T-cell factor mRNA abundance increased significantly throughout the experiment and peaked at 12 h. The upregulation of Rh protein, K + channel, Na + /K + -ATPase, V-type H + -ATPase and vesicle associated membrane protein (VAMP) mRNA, together with downregulation of Na + /K + /2Cl − cotransporter mRNA, indicated an adjustment of general branchial ion-/ammonia-regulatory mechanisms. Meanwhile, hemolymph ammonia concentration was significantly increased in most ammonia-N exposure groups. Histological investigation revealed the hepatopancreatic damage caused by ammonia-N. Results suggest that hormones, biogenic amines and Wnt/β-catenin play a principal role in adapting to ammonia-N exposure and facilitating ammonia transport.

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“…These pathways are implicated in controlling ecdysteroidogenesis in the insect PG ( 74 , 97 , 98 , 205 ) and are well represented in the YO transcriptome ( Table 1 ). Decapods express a large number of Wnt ligands ( 206 ). Wnt4 is implicated in having roles in limb regeneration and the immune response in decapods ( 207 , 208 ).…”

Section: Conclusion and Future Researchmentioning

confidence: 99%

Signaling Pathways That Regulate the Crustacean Molting Gland

Mykles

2021

Front. Endocrinol.

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A pair of Y-organs (YOs) are the molting glands of decapod crustaceans. They synthesize and secrete steroid molting hormones (ecdysteroids) and their activity is controlled by external and internal signals. The YO transitions through four physiological states over the molt cycle, which are mediated by molt-inhibiting hormone (MIH; basal state), mechanistic Target of Rapamycin Complex 1 (mTORC1; activated state), Transforming Growth Factor-β (TGFβ)/Activin (committed state), and ecdysteroid (repressed state) signaling pathways. MIH, produced in the eyestalk X-organ/sinus gland complex, inhibits the synthesis of ecdysteroids. A model for MIH signaling is organized into a cAMP/Ca2+-dependent triggering phase and a nitric oxide/cGMP-dependent summation phase, which maintains the YO in the basal state during intermolt. A reduction in MIH release triggers YO activation, which requires mTORC1-dependent protein synthesis, followed by mTORC1-dependent gene expression. TGFβ/Activin signaling is required for YO commitment in mid-premolt. The YO transcriptome has 878 unique contigs assigned to 23 KEGG signaling pathways, 478 of which are differentially expressed over the molt cycle. Ninety-nine contigs encode G protein-coupled receptors (GPCRs), 65 of which bind a variety of neuropeptides and biogenic amines. Among these are putative receptors for MIH/crustacean hyperglycemic hormone neuropeptides, corazonin, relaxin, serotonin, octopamine, dopamine, allatostatins, Bursicon, ecdysis-triggering hormone (ETH), CCHamide, FMRFamide, and proctolin. Contigs encoding receptor tyrosine kinase insulin-like receptor, epidermal growth factor (EGF) receptor, and fibroblast growth factor (FGF) receptor and ligands EGF and FGF suggest that the YO is positively regulated by insulin-like peptides and growth factors. Future research should focus on the interactions of signaling pathways that integrate physiological status with environmental cues for molt control.

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Wnt gene family members and their expression profiling in Litopenaeus vannamei

Cited by 37 publications

References 35 publications

Genome-Wide Identification and Expression Profiling of Wnt Family Genes in the Silkworm, Bombyx mori

Genome-Wide Identification and Expression Profiling of Wnt Family Genes in the Silkworm, Bombyx mori

Ammonia-N exposure alters neurohormone levels in the hemolymph and mRNA abundance of neurohormone receptors and associated downstream factors in the gills ofLitopenaeus vannamei

Signaling Pathways That Regulate the Crustacean Molting Gland

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