The host response to the low pathogenic avian influenza (LPAI) H5N2, H5N3 and H9N2 viruses were examined in A549, MDCK, and CEF cells using a systems-based approach. The H5N2 and H5N3 viruses replicated efficiently in A549 and MDCK cells, while the H9N2 virus replicated least efficiently in these cell types. However, all LPAI viruses exhibited similar and higher replication efficiencies in CEF cells. A comparison of the host responses of these viruses and the H1N1/WSN virus and low passage pH1N1 clinical isolates was performed in A549 cells. The H9N2 and H5N2 virus subtypes exhibited a robust induction of Type I and Type III interferon (IFN) expression, sustained STAT1 activation from between 3 and 6 hpi, which correlated with large increases in IFN-stimulated gene (ISG) expression by 10 hpi. In contrast, cells infected with the pH1N1 or H1N1/WSN virus showed only small increases in Type III IFN signalling, low levels of ISG expression, and down-regulated expression of the IFN type I receptor. JNK activation and increased expression of the pro-apoptotic XAF1 protein was observed in A549 cells infected with all viruses except the H1N1/WSN virus, while MAPK p38 activation was only observed in cells infected with the pH1N1 and the H5 virus subtypes. No IFN expression and low ISG expression levels were generally observed in CEF cells infected with either AIV, while increased IFN and ISG expression was observed in response to the H1N1/WSN infection. These data suggest differences in the replication characteristics and antivirus signalling responses both among the different LPAI viruses, and between these viruses and the H1N1 viruses examined. These virus-specific differences in host cell signalling highlight the importance of examining the host response to avian influenza viruses that have not been extensively adapted to mammalian tissue culture.
Multiple studies have shown that diabetes mellitus is an established risk factor for periodontitis. Recently mesenchymal stem cells derived from periodontal ligament (PDLSCs) have been utilized to reconstruct tissues destroyed by chronic inflammation. However, impact of periodontitis with diabetes mellitus on PDLSCs and mechanisms mediating effects of complex microenvironments remain poorly understood. In this study, we found multiple differentiation potential of PDLSCs from chronic periodontitis with diabetes mellitus donors (D-PDLSCs) was damaged significantly. Inhibition of NF-κB signaling could rescue osteogenic potential of PDLSCs from simple chronic periodontitis patients (P-PDLSCs), whereas did not promote D-PDLSCs osteogenesis. In addition, we found expression of DKK1 in D-PDLSCs did not respond to osteogenic signal and decreased osteogenic potential of D-PDLSCs treated with DKK1 could be reversed. To further elucidate different character between P-PDLSCs and D-PDLSCs, we treated PDLSCs with TNF-α and advanced glycation end products (AGEs), and find out AGEs which enhance effect of TNF-α in PDLSCs might mediate special personality of D-PDLSCs. The adverse effect of AGEs in PDLSCs could be reversed when PDLSCs were treated with DKK1. These results suggested DKK1 mediating WNT signaling might be a therapy target to rescue potential of PDLSCs in periodontitis with diabetes mellitus.
The frequent occurrence of urban waterlogging constantly affects resident living and urban construction. Improved adaptive prevention and control strategies are highly requested due to huge economic losses and casualties caused by flood and waterlogging in China. The urban waterlogging may evolve into a serious emergency, generally characterized by high complexity, uncertainty, and time pressure. Coupled with the asymmetric information, waterlogging often exacerbates the impact of urban rainstorm disasters. Through the multi-agent system simulation with given geographic information, government and residents interact under dynamic risk distribution in rainstorm disaster. The results show that the proactive attitude of residents and the government towards disaster relief could have a promoting effect for both, thereby increasing the disaster relief efficiency. Obviously, rapid accurate information collection and analysis facilitate disaster relief to a large extent. Meanwhile, appropriate supply rather than excessive supply may mobilize residents’ self-help and balance replenishment of relief supplies.
Abstract:The paper reports on an experimental research study aiming at investigating the effectiveness of employing the continuation tasks in developing EFL student' pragmatic writing skills. To assess the actual effect of the continuation tasks as a predictor of target language pragmatic competence on writing proficiency, the author proposes a task-based approach to achieve pragmatic appropriateness in writing. It is conducted over two pairs of undergraduate students, of whom one pair is regarded as the control group exposed to the traditional writing, the other as the experimental group exposed to the continuation tasks. Procedures are guided by a task-based framework involving stages: 1) pre-task, 2) task cycle, 3) language focus. Measurement tools include: 1) questionnaires of Pragmatic Writing Skills in the pre & follow-up test; 2) the assessment & scores of pre, post & follow-up writings; 3) interviews. Results indicate the continuation tasks' contributing to more appropriate use of target language pragmatic features in writing. Thus the pedagogical implications of this study suggest providing opportunities for language learners to have interaction with target language writers in foreign language contexts through the continuation tasks.
By using the visual analysis software CiteSpace 5.8.R3 and the bibliometric method, this paper presents a statistical analysis of the relevant papers on the application of Chinese intercultural education in CNKI from 2001 to 2021. It reviews papers' publication date, published journals, published institutions, core authors and the focus of keyword clustering, etc. This helps to clarify the current knowledge structure and development context of Chinese intercultural education research, with a view to providing reference for further research in this field in the future.
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