Knowledge of the interactions between nanoparticles (NPs) and cell membranes is of great importance for the design of safe and efficient nanomedicines.
It is often assumed that a hydrophobic drug will be entrapped in the hydrophobic environment of a micelle. Little attention is usually drawn to the actual location of the drug and the effect of the drug on properties. In this publication, we show how the chosen drug curcumin is not only unexpectedly located in the shell of the micelle but also that the accumulation in the hydrophilic block can lead to changes in morphology during self-assembly. A block copolymer poly(1-O-methacryloyl-β-D-fructopyranose)-b-poly(methyl methacrylate), poly(1-O-MAFru) 36 -b-PMMA 192 , was loaded with different amounts of curcumin. The resulting self-assembled nanoparticles were analyzed using transmission electron microscopy, small-angle X-ray scattering (SAXS), and small-angle neutron scattering (SANS). Initial microscopy evidence revealed that the presence of the drug induces morphology changes from cylindrical micelles (no drug) to polymersomes, which decreased in size with increasing amount of drug. SAXS and SANS analysis, supported by fluorescence studies, revealed that the drug is interacting with the glycopolymer block. The drug influenced not only the shape of the drug carrier but also the level of hydration of the shell. Increasing the amount of drug dehydrated the nanoparticle shell, which coincided with a lower nanoparticle uptake by MCF-7 breast cancer cells and noncancerous RAW 264.7 cells. As a result, we showed that the drug can influence the behavior of the nanoparticle in terms of shape and shell hydration, which could influence the performance in a biological setting. Although the depicted scenario may not apply to every drug carrier, it is worth evaluation if the drug will interfere in unexpected ways.
The conjugation of hydrophilic low‐fouling polymers to therapeutic molecules and particles is an effective approach to improving their aqueous stability, solubility, and pharmacokinetics. Recent concerns over the immunogenicity of poly(ethylene glycol) has highlighted the importance of identifying alternative low fouling polymers. Now, a new class of synthetic water‐soluble homo‐fluoropolymers are reported with a sulfoxide side‐chain structure. The incorporation of fluorine enables direct imaging of the homopolymer by 19F MRI, negating the need for additional synthetic steps to attach an imaging moiety. These self‐reporting fluoropolymers show outstanding imaging sensitivity and remarkable hydrophilicity, and as such are a new class of low‐fouling polymer for bioconjugation and in vivo tracking.
Fructose transporter GLUT5 is overexpressed in breast cancer cell lines, but not in healthy tissue. Micelles based on fructose, which were found to be low fouling, showed a high uptake by breast cancer cells (MCF-7 and MDA-MB-231 cells), but only negligible uptake by macrophages.
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