Entry of nanoparticles into cells: the importance of nanoparticle properties

Zhao, Jiacheng; Stenzel, Martina H.

doi:10.1039/c7py01603d

Cited by 308 publications

(277 citation statements)

References 201 publications

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“…Shapeand surface-anisotropic colloids [1] are currently in high demand, as their directional properties are beneficial for hierarchical self-assembly, [2] locomotion, [3] active structuring, [4] interfacial activity, [5] and drug delivery. [6,7] Amongst the conceivable shapes,b owl or cup-shaped particles are as pecial case that combines an anisotropic shape with an open cavity.O wing to their shape,g old nanocups show multiple plasmon resonances and serve as metallodielectric nanoparticles. [8][9][10][11] Cups demonstrate distinct self-assembly behavior through shape-recognition leading to precise colloidal molecules, [12] 1D self-assemblies, [13] or non-classical packing in colloids crystals.…”

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confidence: 99%

Template‐Free Synthesis and Selective Filling of Janus Nanocups

et al. 2019

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We report on the formation of shape-and surfaceanisotropic Janus nanocups (JNCs) by evaporation-induced confinement assembly (EICA) of ABC triblock terpolymers. During microphase separation in spherical confinement, the triblockt erpolymer spontaneously adopted ah emispherical shape with an inner concentric lamella-lamella (ll) morphology.C ross-linking and disassembly of the microparticles resulted in well-defined JNCs with different chemistry on the inside and outside.B ys ynthesizing polymers with increasing length of the cross-linkable block, we tuned the mechanical stability of the nanocups,w hichi sr elevant to control opening and closing of the cup cavity.Weutilize the Janus properties for selective uptake of cargo exemplified by the filling of JNCs with polymer or gold nanoparticles.The directional properties of JNCs suggest applications in locomotion, oil-spill recovery, storage and release,t emplating,a nd as nanoreactors with attoliter volume. Angewandte ChemieCommunications 7123

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confidence: 99%

Template‐Free Synthesis and Selective Filling of Janus Nanocups

et al. 2019

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“…[14][15][16] In contrast, soft nanospheres tend to generate large curvatures at the spreading front that makes nanosphere encapsulation less likely. [20][21] Smooth OMVs are stiffer than rough OMVs, and consequently, they are more likely to achieve full host membrane wrapping by slowly forcing the bilayer to adhere to their round surface.…”

Section: Resultsmentioning

confidence: 99%

To infect or not to infect: molecular determinants of bacterial outer membrane vesicle internalization by host membranes

Jefferies

Khalid

2019

Preprint

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Outer membrane vesicles (OMVs) are spherical liposomes that are secreted by almost all forms of Gram-negative bacteria. The nanospheres contribute to bacterial pathogenesis by trafficking molecular cargo from bacterial membranes to target cells at the host-pathogen interface. We have simulated the interaction of OMVs with host cell membranes to understand why OMV uptake depends on the length of constituent lipopolysaccharide macromolecules. Using coarse-grained molecular dynamics simulations, we show that lipopolysaccharide lipid length affects OMV shape at the host-pathogen interface: OMVs with long (smooth-type) lipopolysaccharide lipids retain their spherical shape when they interact with host cell membranes, whereasOMVs with shorter (rough-type) lipopolysaccharide lipids distort and spread over the host membrane surface. In addition, we show that OMVs preferentially coordinate domain-favoring ganglioside lipids within host membranes to enhance curvature and affect the local lipid composition. We predict that these differences in shape preservation affect OMV internalization on long timescales: spherical nanoparticles tend to be completely enveloped by host membranes, whereas low sphericity nanoparticles tend to remain on the surface of cells.

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“…Studying the interaction between elongated nanoparticles and cells can give us insight on how to design the optimal nanocarrier. Additionally, a compartmentalized structure can potentially be exploited in the co‐delivery of different therapeutic drugs . For these reasons it is important to develop biocompatible triblock copolymer systems that can form hierarchical structures in pure aqueous solution—which continues to be a challenge for triblock terpolymer self‐assembly.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, a compartmentalized structure can potentially be exploited in the co-delivery of different therapeutic drugs. [6] For these reasons it is important to develop biocompatible triblock copolymer systems that can form hierarchical structures in pure aqueous solution-which continues to be a challenge for triblock terpolymer self-assembly. Polymers with pH-responsive blocks can be very useful in this case, and simpler polymer architectures, such as diblock copolymers, have been widely investigated because their solubility can be tuned by changing the acidity of the solution.…”

Section: Introductionmentioning

confidence: 99%

Poly(4‐vinyl imidazole): A pH‐Responsive Trigger for Hierarchical Self‐Assembly of Multicompartment Micelles Based upon Triblock Terpolymers

Piloni

Cao

Garvey

et al. 2019

Macro Chemistry & Physics

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Poly(vinyl pyridine) has widely been used as a pH‐responsive polymer to trigger changes in self‐assembly of block copolymer micelles. However, the polymer is known to display toxic features, which limits its ultimate applicability for biological applications. Here, poly(4‐vinyl imidazole) (P4VIm), a much more biocompatible polymer, is used as a pH‐responsive block to modulate the self‐assembly of ABC triblock terpolymers. In this article, the synthesis of the poly(1‐acryloyl fructopyranose)‐block‐ poly(n‐butyl acrylate)‐block‐ poly(4‐vinyl imidazole) (PFruA52‐b‐PBuA300‐b‐P4VIm250) triblock terpolymers is first discussed by sequential reversible addition fragmentation chain transfer (RAFT) polymerization. Subsequently, the structure formation of the triblock terpolymer is elucidated by step‐wise solvent exchange. The polymer readily dissolves in methanol, but self‐assembles into micelles with PBuA cores and mixed shell in methanol–water mixtures. Solvent exchange against buffer solutions of pH 6–6.5 leads to collapse of P4VIm due to deprotonation and induces self‐assembly into caterpillar‐like non‐spherical nanoparticles, most likely via the formation of intermediate Janus particles. The rearrangement into larger hierarchical structure, as seen by small angle X‐ray scattering (SAXS), is found to process within several hours. The article is concluded by demonstrating lower cytotoxicity values for the present polymer in comparison to a structurally analogous triblock terpolymer based on poly(vinyl pyridine).

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Entry of nanoparticles into cells: the importance of nanoparticle properties

Abstract: Knowledge of the interactions between nanoparticles (NPs) and cell membranes is of great importance for the design of safe and efficient nanomedicines.

Cited by 308 publications

References 201 publications

Template‐Free Synthesis and Selective Filling of Janus Nanocups

Template‐Free Synthesis and Selective Filling of Janus Nanocups

To infect or not to infect: molecular determinants of bacterial outer membrane vesicle internalization by host membranes

Poly(4‐vinyl imidazole): A pH‐Responsive Trigger for Hierarchical Self‐Assembly of Multicompartment Micelles Based upon Triblock Terpolymers

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