AIM: To investigate the role of emodin in protecting the liver against fibrogenesis caused by carbon tetrachloride (CCl4) in rats and to further explore the underlying mechanisms. METHODS:Rat models of experimental hepatic fibrosis were established by injection with CCl4; the treated rats received emodin via oral administration at a dosage of 20 mg/kg twice a week at the same time. Rats injected with olive oil served as a normal group. Histopathological changes were observed by hematoxylin and eosin staining. The activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum and hepatic hydroxyproline content were assayed by biochemical analyses. The mRNA and protein relevant to hepatic stellate cell (HSC) activation in the liver were assessed using real-time reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry, western blotting and enzymelinked immunosorbent assay. RESULTS:The degree of hepatic fibrosis increased markedly in the CCl4 group compared to the normal group (P < 0.01), and decreased markedly in the emodin group compared to the CCl4 group according to METAVIR scale (P < 0.01) compared with those in the normal control group (51.02 ± 10.64 IU/L and 132.28 ± 18.14 IU/L). The activities of serum ALT and AST were significantly higher in rats injected with CCl4 (289.25 ± 68.84 IU/L and 423.89 ± 35.67 IU/L, both P < 0.05).The activities of serum ALT and AST were significantly reduced by administration of emodin (176.34 ± 4 7. 2 9 I U / L a n d 2 2 6 . 1 ± 4 4. 5 2 I U / L , b o t h P < 0.05). Compared with the normal controls (54.53 ± 13.46 mg/g), hepatic hydroxyproline content was significantly higher in rats injected with CCl4 (120.27 ± 28.47 mg/g, P < 0.05). Hepatic hydroxyproline content was significantly reduced in the rats treated with emodin at 20 mg/kg (71.25 ± 17.02 mg/g, P < 0.05).Emodin significantly protected the liver from injury by reducing serum AST and ALT activities and reducing hepatic hydroxyproline content. The mRNA levels of transforming growth factor-β1 (TGF-β1), Smad4 and α-SMA in liver tissues were significantly down-regulated in SD rats that received emodin treatment. Furthermore, significant down-regulation of serum TGF-β1 protein levels and protein expression of Smad4 and α-SMA in liver tissues was also observed in the rats. Emodin inhibited HSC activation by reducing the abundance of TGF-β1 and Smad4. CONCLUSION:Emodin protects the rat liver from CCl4-induced fibrogenesis by inhibiting HSC activation. Emodin might be a therapeutic antifibrotic agent for the treatment of hepatic fibrosis.

Objectives Considering the Western-Eastern cultural differences in parenting practices, as well as the relative paucity of research on the use of mindfulness-based programs by Chinese parents, we replicated a recently proposed Western model of mindfulness. The purpose of this study was to test the direct and indirect relations between parents' dispositional mindfulness, mindful parenting, parenting practices, and child internalizing and externalizing behaviors. Method A total of 2237 Chinses parents (M = 38.46, SD = 4.43) of 6-to 12-year-old children participated in the current study. Results The results showed that parents' dispositional mindfulness was indirectly associated with child internalizing and externalizing behaviors through mindful parenting and positive parenting practices, whereas this pathway was not significant through negative parenting practices. In addition, mothers and fathers demonstrated almost equal effects on direct and indirect pathways except that mothers showed stronger effects on the relationships between dispositional mindfulness and mindful parenting, as well as on the link between negative parenting practices and child externalizing behaviors. Conclusions These findings contribute to a better understanding of the mechanisms underlying how mindfulness and parenting associated with child internalizing and externalizing behaviors, and have important implications for research on interventions aimed at promoting children's psychological well-being. Keywords Mindfulness. Mindful parenting. Internalizing problems. Externalizing problems Mindfulness refers to Bthe awareness that emerges through paying attention, on purpose, in the present moment, and nonjudgmentally to the unfolding of experience moment by moment^(Kabat-Zinn 2003, p. 145). Research has highlighted a number of benefits associated with increased mindfulness, including reduced depression and anxiety symptoms

Loneliness may affect children's psychological well-being and academic achievement. Parent-child relationships have been consistently associated with child adjustment. However, parental antecedents of child loneliness have been relatively understudied. Guided by attachment theory, we examined the trajectories of father-child and mother-child relationship closeness and conflict, and their associations with trajectories of child loneliness with National Institute of Child Health and Human Development (NICHD) Study of Early Child Care and Youth Development (SECCYD) data to understand parents' roles in child loneliness in middle childhood. In Grades 1 ( = 6.57, = .29), 3, 4, and 5, both resident fathers and mothers reported on parent-child relationships. At Grades 1, 3, and 5, children reported on their loneliness. Loneliness declined from Grade 1 to Grade 5. After controlling for socioeconomic status (SES), the slope of father-child relationship closeness was negatively associated with the slope of loneliness for girls ( = 341), but not for boys ( = 354). The more slowly father-child closeness declined, the more quickly girls' loneliness declined. These findings highlight the role of father-child relationships in child loneliness for girls. Future research on child loneliness should take both maternal and paternal roles into consideration. (PsycINFO Database Record

The current study examined parent heart rate (HR) dynamic changing patterns and their links to observed negative parenting (i.e., emotional unavailability and psychological control) during a parent-child conflict resolution task among 150 parent-child dyads (child age ranged from 6 to 12 years, Mage = 8.54 ± 1.67). Parent HR was obtained from electrocardiogram (ECG) data collected during the parent-child conflict resolution task. Negative parenting was coded offline based on the video recording of the same task. Results revealed that emotionally sensitive parents during the task showed greater HR increases while discussing a conflict and greater HR decreases while resolving the conflict, whereas emotionally unavailable parents showed no changes in HR. However, parent psychological control was not associated with HR dynamics during the task. These findings indicated the physiological underpinnings of parent emotional sensitivity and responsiveness during parent-child interactions. The potential association between HR baseline levels and parenting behaviors was also discussed. (PsycINFO Database Record

We examined perceived parental styles, emotion socialization practices and emotion dysregulation among fathers from a community sample in order to understand the intergenerational transmission of normative parenting behaviors. The sample consisted of 217 fathers of school-age children ranging in age from 6 to 12 years. We used a cross-sectional design. The fathers completed a set of self-report questionnaires including parental boding instrument, difficulties in emotion regulation scale, and coping with children's negative emotion scale. The findings indicated that fathers' perceived parental care of their own parents (G1) was negatively linked to fathers' (G2) non-supportive responses to negative emotions shown by their children (G3) through its negative association with the father's (G2) emotion regulation difficulties, whereas fathers' perceived parental overprotection of their own parents (G1) was positively linked to fathers' (G2) nonsupportive responses to negative emotions shown by their children (G3) through its positive associations with the fathers' (G2) emotion regulation difficulties. The current study contributed to the further understanding of individual differences in fathers' emotion socialization practices. The findings potentially shed lights on prevention and intervention efforts regarding limiting the expansion of maladaptive emotional parenting behaviors across generations.

Using a family systems perspective, we examined the trajectories of father-child and mother-child closeness and conflict across Grades 1, 3, 4, 5, and 6, and their associations with child depressive symptoms across middle childhood among 685 families in the Eunice Kennedy Shriver National Institute of Child Health and Human Development Study of Early Child Care and Youth Development (SECCYD). Father-child and mother-child relationship conflict increased, whereas relationship closeness decreased from Grades 1 to 6. Girls with more slowly increasing father-child conflict, and more slowly decreasing father-child closeness, were at lower risk for depressive symptoms. Boys with more slowly increasing mother-child conflict were at lower risk for depressive symptoms. These findings highlight the important roles of both father-child and mother-child relationships in children's emotional adjustment during middle childhood.

Although the association between maternal gatekeeping and relationship functioning has been explored by a few studies, none of these have focused on fathers' perceptions of these constructs. Given that today's new fathers are challenged by elevated expectations for active parenting and coparenting even as most new mothers remain primary caregivers of infant children, this is a critical omission. This study examined the associations between new fathers' perceptions of maternal gatekeeping and change in dyadic adjustment as mediated through coparenting closeness. Maternal gatekeeping was reported by 182 fathers at 3 months postpartum, coparenting closeness was reported at 3 and 6 months postpartum, and dyadic adjustment was reported during the third trimester of pregnancy and at 9 months postpartum. Fathers' perceptions of relative change in coparenting closeness from 3 to 6 months mediated associations between fathers' perceptions of maternal gatekeeping at 3 months and relative change in dyadic adjustment from the third trimester to 9 months postpartum. In particular, findings indicate that greater perceived maternal gate opening was associated with higher levels of dyadic adjustment through higher levels of coparenting closeness, whereas greater perceived maternal gate closing was associated with lower levels of dyadic adjustment through lower levels of coparenting closeness. This study highlights the importance of studying fathers in the context of the family system and the role of the coparenting relationship at the transition to parenthood in couple relationship functioning.

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