Aspirin-induced apoptosis is one of the important mechanisms for its antitumour effect against gastric cancer. We aimed at investigating the involvement of bcl-2 family members in the apoptotic pathway in gastric cancer. Gastric cancer cell line AGS and MKN-45 were observed as to cell growth inhibition and induction of apoptosis in response to treatment with aspirin. Cell proliferation was measured by MTT assay. Apoptosis was determined by 4'-6-diamidino-2-phenylindole staining. Protein expression was determined by western blotting. We showed that aspirin activated caspase-8, caspase-9 and capase-3, cleaved and translocated Bid, induced a conformational change in and translocation of Bax and cytochrome c release. In addition, suppression of caspase-8 with the specific inhibitor z-IETD-fmk, as well as the pan-caspase inhibitor z-VAD-fmk, prevented Bid cleavage and subsequent apoptosis. The caspase inhibitors failed to abolish the effects on Bax activation. In conclusion, our results identify a role of caspase-8/Bid and activation of Bax as a novel mechanism for aspirin-induced apoptosis in gastric cancer.

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Silver doped LiFePO 4 powder was synthesized using the hydrothermal method, and was used as a sensing material. An Optical WaveGuide (OWG) sensor which reacted to Volatile Organic Compound (VOC) was developed by spin-coating a thin film of LiFe 0.99 Ag 0.01 PO 4 onto a Tin-diffused glass optical waveguide. This OWG sensor was used to detect formaldehyde gas as a typical example of VOCs. The experimental result showed that the sensor easily and repeatedly detected 100ppt of formaldehyde, with a short response time (less than 2 s). At the low concentration (below 10ppm), the volatile organic compounds caused no interference with the detection of formaldehyde vapor. The sensor also had the characteristics of high sensitivity, a simple structure, and easy fabrication.

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