Introducción: Indagar acerca del efecto del consumo de frutas enteras sobre la expresión de ARNm de algunos genes inflamatorios en muestras de sangre periférica de jóvenes universitarios colombianos.Materiales y métodos: Previo a la intervención se aplicaron cuestionarios para explorar sobre algunos factores del estilo de vida. En el día 1, todos los voluntarios consumieron una comida de prueba. En los días 2 a 14, los individuos del grupo control continuaron con su alimentación habitual mientras que los del grupo tratamiento consumieron una porción diaria de frutas enteras. Durante la intervención se recolectaron muestras de sangre para determinar los niveles séricos de glucosa, colesterol y triglicéridos, así como para establecer la expresión de ARNm de los genes inflamatorios TNFR, IL1R, IL6R, TLR2, TLR4 y RELA a través de la técnica de qPCR.Resultados: La expresión de ARNm del gen RELA varió según el nivel de estrés auto-reportado por los voluntarios. En los individuos del grupo tratamiento se observó una reducción en la expresión de ARNm de los genes RELA e IL6R junto con un incremento en los niveles séricos de colesterol HDL.Conclusiones: El consumo de frutas enteras parecer ser un factor relevante en la modulación de la expresión de ARNm de los genes RELA e IL6R así como en la regulación de los niveles séricos de colesterol HDL. Adicionalmente, el estrés parece ser otro elemento clave en la modulación de la expresión de ARNm del gen RELA.
Background. Dietary exposure to mercury in women of childbearing age could result in neurological effects on the fetus. A health risk assessment of total mercury by fishery products intake has not been conducted in this population group in Bogota, Colombia. On the other hand, it has been suggested that selenium content on fishery products may have a protective effect against mercury toxicity. Nevertheless, selenium content on fish species marketed in Bogotá has not been determined. Objective. Exposure risk to total mercury and selenium content on fishery products consumed by women of childbearing age from Bogota, Colombia, were assessed. Methods. Total mercury and selenium concentrations for products available at fish stores and supermarkets were determined. The exposure risk to total mercury was estimated considering the intake of these products by women of childbearing age group. Results. Total mercury highest concentrations were 0.8166 mg/kg in mota (Calophysus macropterus), and 0.6275 mg/kg in catfish (Pseudoplatystoma tigrinum). On the other hand, the highest selenium concentration was 0.6471 mg/kg in nicuro (Pimelodus blochii). Finally, it was established that for women of the childbearing age group, health risk of exposure to total mercury due to mota intake exceeded by 8.56-fold the reference dose. Conclusions. Mota intake considerably increases exposure risk to total mercury on women of childbearing age from Bogota, Colombia. The selenium levels established in the fishery products assessed, except for catfish and mota, are theoretically suggestive of a protective effect of selenium against mercury toxicity. Consequently, continuous total mercury concentrations monitoring is required to protect health of the women of childbearing age and the general population from Bogota, Colombia.
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