Theory predicts, and some evidence demonstrates that in lakes, the depth of the thermocline can have a large structural influence on the spatial distribution, and strongly influences the composition of plankton communities. However, experimental assessments of responses of the planktonic food web to thermocline depth have not yet been done at the whole-basin scale. We conducted an experiment wherein we artificially lowered the thermocline in an isolated basin of a three-basin lake, maintaining another isolated basin as a control. The vertical distribution and taxonomic composition of both phytoplankton and zooplankton were monitored throughout the summer months. Greater phytoplankton production, especially in the epilimnion, attributable mainly to increases in the chlorophytes was observed with thermocline deepening, but at the deepest thermoclines, production was limited. Total zooplankton biomass was unaffected by thermocline depth, suggesting top-down control by predators. Zooplankton biomass peaks were less pronounced in the manipulated basin, but tended to follow the thermocline whether at its normal position or as it was deepened. Zooplankton composition was significantly altered by large increases in densities of predatory cyclopoid copepods and rotifers; taxa commonly found in turbulent environments. Overall, both phytoplankton and zooplankton communities demonstrated important shifts in structure and composition in response to thermocline deepening.
Patterns of diatom species distribution in relation to total N (TN), total P (TP), and other environmental variables from riffle sites on 2 streams in southern Ontario, Canada, were determined using canonical correspondence analysis (CCA). Relationships with TN and TP were sufficiently strong to develop weighted-averaging (WA) regression-calibration models for inferring stream water concentrations of these nutrients. The models were accurate within Ϯ2.4 g/L for TP (apparent r 2 ϭ 0.52) and Ϯ2 mg/L for TN (apparent r 2 ϭ 0.53). An evaluation of the goodness of fit of these models with and without bootstrapping indicated that they performed better (bootstrapped r 2 ϭ 0.44 for TP and bootstrapped r 2 ϭ 0.42 for TN) than published TN and TP inference models for which similar assessments were made. Based on Organisation for Economic Cooperation and Development eutrophication ratings, the TP model predicted 76% of the mesotrophic and 57% of the eutrophic samples correctly. The model correctly predicted only 20% and 33%, respectively, of oligotrophic and hypereutrophic samples. WA inference models were improved when seasonal variation was removed by using mean summer water quality and diatom data (apparent r 2 ϭ 0.76 and bootstrapped r 2 ϭ 0.61 for TP; apparent r 2 ϭ 0.82 and bootstrapped r 2 ϭ 0.70 for TN). Overall, we conclude that epilithic diatoms can be related to TN and TP using these methods, and that WA inference models have utility for indicating eutrophication in southern Ontario lowland streams.
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