This study describes the in vitro anthelmintic activity of extracts from Opuntia ficus indica against gastrointestinal nematodes of sheep. The anthelmintic activity was evaluated by inhibition of egg hatching, larval development and larval migration assays. The residual aqueous fractions from cladodes and fruits showed higher ovicidal activity with EC50 values of 7.2mg/mL and 1.5mg/mL, respectively. The aqueous, hexane, and ethyl acetate fractions from fruits and the aqueous fraction from cladodes inhibited 100% of larval development at the lowest concentration tested (1.56mg/mL). The crude cladode and fruit ethanolic extracts inhibited larval migration and showed EC50 values of 0.74mg/mL and 0.27mg/mL, respectively. Phytochemical screening detected high concentrations of alkaloids, tannins, flavonoids, and saponins in the fruits and cladodes. The results demonstrated that O. ficus exhibits anthelmintic activity in vitro, suggesting that, beyond its nutritional potential, this plant can also be an ally for parasite control in sheep.

The objective with this study was to calculate the total cost of maize production and beef cattle in permanent pasture activities in separate production systems (conventional) and integrated systems (maize production plus beef cattle), as well as to verify economic gains explained by the economy of scope. The first step for the development of the research was to obtain field experimental data. Six experimental treatments were studied: corn grain production, beef cattle in permanent pasture, and four integrated systems based on different crop and pasture sowings. The second step consisted in the collection, calculation, and allocation of variable and fixed costs to estimate costs of production in the systems. The crop-livestock integration showed economic gains in relation to conventional systems, which can be explained by the dilution of fixed costs and the presence of shareable inputs, resulting in economy of scope. It was also possible to demonstrate that total unit costs of both crop and livestock were lower in crop-livestock integration, showing that integrated systems resulted in economic benefits as compared with conventional ones.

The objective of this study was to evaluate the potential of improving the economic value of integrated crop-livestock systems (ICLS) compared to conventional systems specialized in monoculture. The experimental area was 16.02 ha, divided into 18 paddocks of 0.89 ha each, organized in a randomized block design, with three replicates and six models of production systems: crop system [corn (Zea mays) grain production], livestock system (beef cattle under grazing conditions), and four ICLS, identified as: ICLS-1, corn integrated with Marandu palisadegrass [Urochloa brizantha (Hoechst. ex A. Rich.) R.D. Webster cv. Marandu (syn. Brachiaria brizantha cv. Marandu] sown simultaneously without herbicide; ICLS-2, corn and Marandu palisadegrass sown simultaneously with herbicide; ICLS-3, corn and Marandu palisadegrass with lagged sowing; and ICLS-4, corn and Marandu palisadegrass sown simultaneously, with herbicide in rows and between-rows of corn. We demonstrated the economic impact analysis combined with the risk optimization and discounted cash flow techniques based on Monte Carlo simulation, considering price and productivity uncertainties. The indicators of added value and return on investment of ICLS had an economic advantage compared with conventional systems. It was also found that ICLS needed a smaller operational area than conventional systems for the economic break-even point. Integrated systems provide lower financial and operational risk levels and greater economic value per hectare compared with conventional systems specialized in monoculture.

Soil quality indicators may have different seasonal trends during the year. The hypothesis of this study was that sowing maize and marandu palisadegrass with the presence of grazing animals might cause short-term changes in soil indicator responses. We compared four intercropping techniques: ICLS-1: maize plus marandu palisadegrass sown simultaneously, without herbicide; ICLS-2: maize plus marandu palisadegrass sown simultaneously, with herbicide; ICLS-3: maize plus marandu palisadegrass in lagged sowing (i.e., marandu palisadegrass sown 20 days after maize sowing); ICLS-4: maize plus marandu palisadegrass sown simultaneously (palisadegrass on the rows and inter-rows, with herbicide). Two control treatments in monoculture (i.e., grass and maize monocultures) represented the conventional systems. The soil was sampled three times over a one-year period, for the evaluation of carbon and nitrogen fractions as affected by seasonal variability. Results indicated that ICLS-1 and ICLS-4 enhanced soil quality more quickly during the seasonal variation.The ICLS-2 negatively affected microbial biomass (22%), resulting in reduced labile carbon (43%), carbon management index (33%), and potentially mineralizable nitrogen (20%). Labile carbon and the carbon management index were more sensitive and efficient indicators for verifying soil quality changes in the short-term. Results indicated that there is large variability in these soil indicators during the annual cycle, and further studies are needed to verify the effects of the tested systems on different soil nitrogen fractions and how they affect available nitrogen in the short-term. However, potentially mineralizable nitrogen can provide positive insights for short-term changes in the soil.

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