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Nitric oxide (NO) is a messenger molecule that is produced in the brain from the metabolism of L-arginine to L-citruline. Growing evidence suggests a physiological role for NO in long-term potentiation (LTP). Since LTP is a form of synaptic plasticity thought to be involved in learning and memory, we have tested whether inhibition of endogenous NO production affects memory capacities ofrats. We found that the NO synthase [L-arginine, NADPH:oxygen oxidoreductase (nitric oxide-forming), EC 1.14.13.39] inhibitor N01-nitro-Larginine, at doses blocking LTP in hippocampal slices, impairs spatial learning in a radial arm maze and olfactory memory in a social recognition test. In contrast, N"-nitro-L-arginine left shock-avoidance learning unaffected. These results indicate that NO is involved in some but not all forms of memory and further support the existence of a causal link between LTP and spatial learning.Nitric oxide (NO) is a diffusible molecule endowed with intercellular messenger properties in several biological systems including the brain (1, 2). NO mediates the stimulation of soluble guanylate cyclase upon activation of N-methyl-Daspartate (NMDA) receptors (3) and serves as its own negative feedback effector by blocking NMDA-evoked responses (4, 5). This messenger is produced from the enzymatic conversion of L-arginine to L-citrulline by a constitutive NO synthase [NOS; L-arginine, NADPH:oxygen oxidoreductase (nitric oxide-forming), EC 1.14.13.39] which can readily be blocked by arginine analogs, such as Nl-nitro-Larginine [Arg(NO2); also called NG-nitro-L-arginine, where G refers to the guanidino-carbon] (6, 7).Long-term potentiation (LTP) is a persistent increase, which can last for days or weeks, in the synaptic efficacy of pathways produced by brief periods of high-frequency stimulations (HFS) (8). This phenomenon, best characterized in the hippocampus, is thought to be a cellular event involved in the acquisition, storage, or retrieval of information in the brain (9-12). We and others reported recently that NOS inhibitors and NO scavengers block hippocampal LTP in rat brain slices (13)(14)(15)(16) to irreversibly block brain NOS enzymatic activity (7). Separate groups of animals were used for each experiment. Vehicle-treated animals were used as controls.Electrophysiology. Sixteen hours after the last injection, transverse hippocampal slices (0.5-mm thick) were prepared from Sprague-Dawley rats (150-200 g) pretreated with Arg(NO2) (25-100 mg/kg of body weight i.p.) or vehicle. Slices were maintained in a submersion-type recording chamber under superfusion (2.5-3 ml/min) with gassed (95% 02/5% C02) medium containing 124 mM NaCl, 5 mM KCl, 2 mM MgSO4, 2 mM CaCl2, 26 mM NaHCO3, 1.25 mM KH2PO4, and 10 mM glucose at 32°C as described (18). Stimulation and recording electrodes were positioned in the CAl-stratum radiatum, and field excitatory postsynaptic potentials (EPSPs) were evoked every 5 s. The stimulus strength (0.1-ms duration at 2-20 V) was adjusted to evoke EPSPs of at least 0.3-mV amplitude w...

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1 The aim of this study was to investigate the e ect of N-(3-(aminomethyl)benzyl)acetamidine (1400W), a selective inhibitor of inducible calcium-independent nitric oxide synthase (iNOS), on the functional and histopathological outcomes of experimental transient focal cerebral ischaemia in rats. 2 Transient ischaemia was produced by the occlusion for 2 h of both the left middle cerebral artery and common carotid artery. Treatments with 1400W (20 mg kg 71 ) or vehicle were started 18 h after occlusion of the arteries and consisted in seven subcutaneous injections at 8 h interval. Ischaemic outcomes and NOS activities (constitutive and calcium-independent NOS) were evaluated 3 days after ischaemia. 3 1400W signi®cantly reduced ischaemic lesion volume by 31%, and attenuated weight loss and neurological dysfunction. 4 1400W attenuated the calcium-independent NOS activity in the infarct by 36% without a ecting the constitutive NOS activity. 5 These ®ndings suggest that iNOS activation contributes to tissue damage and that selective inhibitors of this isoform may be of interest for the treatment of stroke.

Nitric oxide production in the cerebellum and induction of long-term potentiation (LTP) in the hippocampus have some characteristics in common: both phenomena are induced by activation of N-methyl-d-aspartate receptors and both are highly dependent on calcium-mediated processes. Here we provide evidence that endogenous nitric oxide production is necessary for synaptic plasticity in the CA1 hippocampus of the rat. LTP recorded in slices was blocked in a concentration-dependent manner by the nitric oxide synthase inhibitors l-NG-nitroarginine and l-NG-nitroarginine methyl ester, but l-NG-monomethylarginine was only marginally active. Bathing the slices with haemoglobin, a protein that scavenges nitric oxide, also resulted in a concentration-dependent blockade of LTP. Nitric oxide released locally from hydroxylamine produced a stable potentiation of synaptic transmission that was not additive with LTP induced by high-frequency stimulation. These results are fully consistent with the presumed retrograde messenger role of nitric oxide in LTP.

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Brain trauma is the main cause of morbidity and mortality in young adults. One delayed events that occurs after a head trauma and compromises the survival of patients is cerebral edema. The present study examined first the occurrence of cerebral edema after a traumatic brain injury (TBI) induced by moderate fluid percussion in rats. Brain water content was measured from 1 h to 7 days posttrauma, in the hippocampus and cortex, on both ipsi- and contralateral hemispheres. Second, the effects of mannitol, an osmotic agent frequently used in the clinic, and riluzole, a neuroprotective compound, were investigated on regional edema formation. After TBI, the ipsilateral edema began early at 1-6 h, was maximal at 48 h and was resorbed by 5-7 days. No edema was observed in the contralateral hemisphere. Mannitol at 1 g/kg or vehicle was administered iv 15 min, 2 h and 4 h postinjury. At this dose, mannitol significantly attenuated the ipsilateral injured cortex edema measured at 6 h (p < 0.05). Riluzole at 4 and 8 mg/kg or vehicle was administered 15 min (IV) and 6 h, 24 h, and 30 h (SC) post-TBI. Riluzole at 4 x 4 mg/kg significantly reduced edema measured at 48 h, in the ipsilateral hippocampus (p < 0.05), whereas at 4 x 8 mg/kg, the reduction was observed in the hippocampus (p < 0.01) and the injured cortex (p < 0.05). Our results demonstrate that (1) cerebral edema begins early after the injury and is resorbed over 1 week; (2) mannitol could attenuate cerebral edema; and (iii) riluzole in addition to its neuroprotective effects reduces the brain edema. Thus, riluzole could be useful in human TBI treatment.