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Blood and urinary vitamin Be and urinary 4-pyridoxic acid values are reported for 3 rhesus monkeys before and after a 5-day period of pyridoxol-HCl sup plementation. Blood and liver vitamin Be values are given for a group of free-ranging howler monkeys. Microbiological procedures employing Saccharomyces carlsbergensis ATCC 9080, Streptococcus faecium 0 51 NCDO 1229 and Lactobacillus casei ATCC 7469 were used for the vitamin B8 analyses and a microprocedure of the lactone method was used for 4-pyridoxic acid determinations. Large increments of vitamin Be in blood and urine and increased excretion of 4-pyridoxic acid were observed in the rhesus monkeys following supplementation. Of significance was the prolonged elevation of values several days after supplementation had ceased. Blood values for the howler monkeys were higher than those for the rhesus. Differential assay of liver hydrolysates from howler monkeys revealed that most of the vitamin was present in the pyridoxamine form. A lesser amount was in the pyridoxal form and the amount of pyridoxol was negligible.

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