The current study investigated synergism of elevated hydrostatic pressure, habituation, mild heat, and antimicrobials for inactivation of O157 and non-O157 serogroups of Shiga toxin-producing Escherichia coli. Various times at a pressure intensity level of 450 MPa were investigated at 4 and 45 °C with and without carvacrol, and caprylic acid before and after three-day aerobic habituation in blueberry juice. Experiments were conducted in three biologically independent repetitions each consist of two replications and were statistically analyzed as a randomized complete block design study using ANOVA followed by Tukey- and Dunnett’s-adjusted mean separations. Under the condition of this experiment, habituation of the microbial pathogen played an influential (p < 0.05) role on inactivation rate of the pathogen. As an example, O157 and non-O157 serogroups were reduced (p < 0.05) by 1.4 and 1.6 Log CFU/mL after a 450 MPa treatment at 4 °C for seven min, respectively, before habituation. The corresponding log reductions (p < 0.05) after three-day aerobic habituation were: 2.6, and 3.3, respectively at 4 °C. Carvacrol and caprylic acid addition both augmented the pressure-based decontamination efficacy. As an example, Escherichia coli O157 were reduced (p < 0.05) by 2.6 and 4.2 log CFU/mL after a seven-min treatment at 450 MPa without, and with presence of 0.5% carvacrol, respectively, at 4 °C.
In view of the high potential of ricebran oil in India, lecithins recovered from crude and dewaxed Indian ricebran oil were analyzed and different classes characterized with the objective of effectively utilizing this valuable by‐ product. Lipid classes and individual phospholipid components were identified and estimated. Dewaxing was found to have a considerable effect on composition of the derived lecithin. The lecithin obtained from crude or dewaxed Indian ricebran oil consisted mainly of phosphatidylcholine, phosphatidylethanolamine, phosphatidylnositol and triglycerides, along with carbohydrates, free fatty acid, sterols and waxes (in case of crude oil). The major fatty acids of individual phospholipids were found to be palmitic, oleic and linoleic. Analytical characteristics of ricebran lecithin were shown to be comparable to local soybean lecithin. It can be expected that the gummy materials in the oil, presently lost with the soapstock during refining, could find important applications.
Five fatty acids comprise the bulk of the lipid content in pecans: palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acid. Understanding the profiles of these fatty acids and how they relate to sensory characteristics may offer an explanation for flavor and flavor defects that may exist in certain cultivars of pecans. The objective of this study was to examine and compare fatty acid profiles of three cultivars of pecans (Major, Lakota, and Chetopa), over two crop years, under raw and roasted preparation methods, and understand the fatty acids association with sensory attributes. Percentages of palmitic, stearic, oleic, linoleic, and linolenic acids to total fatty acid content were determined using gas chromatography, and sensory profiles were generated using descriptive sensory analysis. Similar trends were seen across samples, with oleic acid comprising the majority of the total fatty acids and linolenic acid comprising the smallest percentage. There were significant differences in fatty acid content among cultivars and between pecans in the first and second crop year. Few associations were found between the fatty acids and sensory attributes, which suggest that combinations of the fatty acids contribute to certain pleasant or undesirable flavor attributes in the pecans. Subtle differences in fatty acid composition may lead to variation in flavor and flavor intensity or draw attention to or from certain attributes during consumption. Differences in crop year indicated that fatty acid content and therefore flavor are variable year to year. Practical Application This study will help understand how fatty acid content of pecans varies from year to year. This should be taken into account when manufacturing products with pecans as the nutritional content of the product may change as the result.
Interesterification of fats is being used increasingly as an alternative to hydrogenation in preparing shortening and margarine bases. The detection of interesterified fats in vanaspati (a hydrogenated fat) is relevant because of possible adulteration problems. Either palmitic acid‐rich or stearic acid‐rich interesterified fats were blended with 13 market samples of hydrogenated fat (vanaspati) and examined by on‐plate lipase hydrolysis of glycerides, gas chromatographic determination of fatty acids of the isolated 2‐monoglycerides and calculation of two emperical indices. These were R1, the ratio of the amounts of palmitic acid present in the 2‐position to that in the total glyceride, and R2, the ratio of saturated acid present in the 2‐position to total saturated fatty acid in the fat. The vanaspati, R1 was always below 10 and R2 was always below 20. The presence of 5–10% interesterified fat raised both figures and offered a suitable basis for the detection of interesterified fats in hydrogenated fats.
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