Type I collagen from outer skin of Sepia pharaonis was extracted and partially characterized. Yield of Acid Soluble Collagen (ASC) and Pepsin Soluble Collagen (PSC) were calculated as 1.66% and 3.93% and the total protein content of ASC and PSC were found as 18.4% and 48.6%. FT-IR spectrum of ASC and PSC recorded 12 and 14 peaks, respectively. 1H NMR spectrum of ASC showed singlets at 1.23 ppm, 3.1 ppm, 3.55 ppm and 3.7 ppm and PSC at 1.23 ppm and 2.08 ppm. The molecular weight for ASC was calculated as 102 kDa and for PSC as 110, 108 and 102 kDa through SDS-PAGE. Differential Scanning Calorimetry (DSC) results supported that PSC withstand high thermal stability (82.85 °C) than ASC (73.13 °C). Higher denaturation temperature with high molecular weight well support the property of type I collagen from skin of S. pharaonis and it could be used as another potent source for the extraction of collagen.
The need for the discovery of new and novel antibiotics is imperative because evidence suggests that development and spread of resistance to any new antimicrobial agent is inevitable. In the present study, the in vitro antibacterial activity of methanolic extract of whole body tissue and ethylene diamine tetra acetate (EDTA) extract of cuttlebone (polysaccharide) of Sepia pharaonis was investigated against ten bacterial species including Gram-positive species (Staphylococcus aureus and Streptococcus pyogenes) and Gram-negative species (Salmonella typhi, Klebsiella pneumoniae, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Vibrio parahaemolyticus and Proteus mirabilis) with different concentrations such as 25, 50, 75 and 100% using disc diffusion method. The highest inhibition zone was recorded against P. mirabilis for methanolic extract (18.3±0.1 for 100% concentration) and against S. pyogenes for EDTA extract (polysaccharide) (15.5±0.06 for 100% concentration) of cuttlebone. But the activity was totally absent in negative control. For minimum inhibitory concentration (MIC) technique, various ranges of concentrations between 20 and 100 mg/ml were prepared and tested. MIC values were found ranging from 40 and 100 mg/ml. All assays were carried out in triplets. A wide spectral and concentration dependent antibacterial activity was recorded in both extracts.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.