SUMMARYChanna punctatus was exposed to four different concentrations of Rutin, Taraxerol and Apigenin. Changes in some hematological parameters of Channa punctatus were assessed to determine the influence of these compounds on test fish. Fish were exposed to sublethal concentrations (80% of LC 50 of 24h) of these compounds for one week. Control fish were also administered for one week. Thereafter, blood samples were obtained from the control and experimental fish. Blood was assayed for selected hematological parameters (hematocrit, hemoglobin, red blood cell count, white blood cell count total plasma protein and plasma glucose concentration). The derived hematological indices of mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH) and mean corpuscular volume (MCV) were calculated. Sublethal concentrations of these compounds caused a dose dependent decrease in hemoglobin values coupled with a decrease in hematocrit values and red blood cell counts are an obvious indication of anemia. The total white blood cell counts and the differential white blood cell counts were decreased except for the lymphocytes, where there was a slight increase. Plasma protein and glucose were also lower in exposed fish when compared with control. The hematological indices MCH, MCHC, MCV were also lowered. The result from this study reveals high mortality rate and deleterious consequences on the health of fish subjected to acute exposure of Rutin, Taraxerol and Apigenin and therefore, should not be used directly in aquaculture without having the proper knowledge.

ABSTRACT-The present study deals with the physicochemical characteristics of river water Aami. A pulp and paper mill namely Rayana Paper Board Industries Ltd Khalilabad, Uttar Pradesh, were studied for sample collection and analysis of various pollution parameters. Three sampling sites have been selected for this study. Water of this river has toxic effects on fish Channa punctatus. The 96h LC 50 values of these sites are 8.99 % dilution for site 1, 22.96 % dilution for site 2 and 41.15 % dilution for site 3. Fishes were treated with different sub-lethal doses of water samples, it shown significant alterations in different biochemical and haematological parameters of fish.