Effect of bioactive compounds extracted from euphorbious plants on hematological and biochemical parameters of Channa punctatus

Abstract

SUMMARYChanna punctatus was exposed to four different concentrations of Rutin, Taraxerol and Apigenin. Changes in some hematological parameters of Channa punctatus were assessed to determine the influence of these compounds on test fish. Fish were exposed to sublethal concentrations (80% of LC 50 of 24h) of these compounds for one week. Control fish were also administered for one week. Thereafter, blood samples were obtained from the control and experimental fish. Blood was assayed for selected hematological parameters (hematocrit, hemoglobin, red blood cell count, white blood cell count total plasma protein and plasma glucose concentration). The derived hematological indices of mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH) and mean corpuscular volume (MCV) were calculated. Sublethal concentrations of these compounds caused a dose dependent decrease in hemoglobin values coupled with a decrease in hematocrit values and red blood cell counts are an obvious indication of anemia. The total white blood cell counts and the differential white blood cell counts were decreased except for the lymphocytes, where there was a slight increase. Plasma protein and glucose were also lower in exposed fish when compared with control. The hematological indices MCH, MCHC, MCV were also lowered. The result from this study reveals high mortality rate and deleterious consequences on the health of fish subjected to acute exposure of Rutin, Taraxerol and Apigenin and therefore, should not be used directly in aquaculture without having the proper knowledge.

Effects of Pesticides on Haematology, Thyroid Stimulating Hormone (TSH) and Tri-iodothyronine (T3) Hormones of Agricultural Workers in Swat, Pakistan

“…Interestingly, WBC and PLT were the most affected parameters (Table 1). Shahi and Singh (2011) notions are also in agreement with the current findings who reported an increase in the WBC count in response to the pesticides exposure. Similarly, an increase in the levels of Mid %, Gran %, MCV and WBC has also been reported by Dunstan et al (1996), which again justify our findings.…”

Automatic analysis to assess haematological parameters in farmed gilthead sea bream (Sparus aurataLinnaeus, 1758)

“…Tavares-Dias et al (2008) used an automatic blood cell counter for the evaluation of red blood cells (RBC) in two neotropical freshwater teleost species, but all other haematological parameters such as white blood cells (WBC), and thrombocyte counts (TC) were assessed using blood smear techniques. Shahi and Singh (2011) reported that they used an auto analyser to measure parameters such as hematocrit value (PCV), hemoglobin content (Hb), WBC, and RBC in the specimens of Channa punctatus; as they supplied little details of their method, it is difficult to assess the comparability of the values reported.…”

Efficacy of Achyranthes aspera (L.) as a Molluscicidal Bait Formulation against Fresh Water Snail Biomphalaria pfeifferi

“…Parallel to the search for noble molluscicides of higher efficacy and safety, adoption of efficient application mechanisms could play a decisive role in enhancing their effectiveness. In principle, such molluscicides should be applied in dose as low as possible for environmental, natural resource conservation and economic reasons [ 9 , 10 ]. In the usual immersion method, the molluscicide is added to the water habitat [ 11 , 12 ] and is needed in large amount to titrate the entire water body.…”