Bone is a dynamic tissue that constantly adapts to changing mechanical demands. The transforming growth factor beta (TGFβ) signaling pathway plays several important roles in maintaining skeletal homeostasis by both coupling the bone‐forming and bone‐resorbing activities of osteoblasts and osteoclasts and by playing a causal role in the anabolic response of bone to applied loads. However, the extent to which the TGFβ signaling pathway in osteocytes is directly regulated by fluid shear stress (FSS) is unknown, despite work suggesting that fluid flow along canaliculi is a dominant physical cue sensed by osteocytes following bone compression. To investigate the effects of FSS on TGFβ signaling in osteocytes, we stimulated osteocytic OCY454 cells cultured within a microfluidic platform with FSS. We find that FSS rapidly upregulates Smad2/3 phosphorylation and TGFβ target gene expression, even in the absence of added TGFβ. Indeed, relative to treatment with TGFβ, FSS induced a larger increase in levels of pSmad2/3 and Serpine1 that persisted even in the presence of a TGFβ receptor type I inhibitor. Our results show that FSS stimulation rapidly induces phosphorylation of multiple TGFβ family R‐Smads by stimulating multimerization and concurrently activating several TGFβ and BMP type I receptors, in a manner that requires the activity of the corresponding ligand. While the individual roles of the TGFβ and BMP signaling pathways in bone mechanotransduction remain unclear, these results implicate that FSS activates both pathways to generate a downstream response that differs from that achieved by either ligand alone.
The blood-flesh peach or vineyard peach is an older heritage cultivar with juicy red-flesh and tart-sweet flavor. They are popular in France, where more than 200 years ago wine growers used to plant them on the vineyards as biological markers to detect the presence of powdery mildew. It is present in countries such as China, Italy, New Zealand, Australia and USA however, it remains a very rare variety worldwide. In Chile, the blood-flesh peach has a centenary presence in rural orchards where is called “Durazno Betarraga.” Reproduced by seeds, it has pass through generations of family farmers and has been adapted to local environmental conditions. This red-flesh peach is a local variety considered part of their traditional diets, however, cultural changes in food consumption, short postharvest life and water scarcity due to climate change are threatening its conservation. One of the objectives of the International Year of Fruits and Vegetables, as defined by the FAO, is to integrate small holders and family farmers into value chains for sustainable production and consumption of fruits and vegetables recognizing the contributions of farmer's landraces to their food security, nutrition, livelihoods and income. To promote this objective, we present the work we have been carry out for several years with a farming community. We have conducted ethnographic research to provide a qualitative description of the agricultural value of the blood peach in a limited territory of the Maule Region defined as the study area. For the quantitative section of our research we analyzed the antioxidant capacity (ORAC) and total polyphenol content and compared them with those of other fruits. To gather information on the presence of the blood-fleshed peach in other regions of Chile, we used a citizen science approach through social networks. We propose that this local variety is an innovative raw material to develop healthy fruit-based food, thus encouraging its conservation and consumption with a positive social and economic impact for the community and the local food system.
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