Enzymatic production of lactulose by fed-batch and repeated fed-batch reactor

Ramírez, Nicolás; Ubilla, Claudia; Campos, Javiera; Valencia, Francisca; Aburto, Carla; Vera, Carlos; Illanes, Andrés; Guerrero, Cecilia

doi:10.1016/j.biortech.2021.125769

Cited by 16 publications

(3 citation statements)

References 33 publications

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“…Subsequently, the biocatalyst was separated from the broth by means of centrifugation and the recovered pellet was employed as a biocatalyst in the next batch run. The main advantage of repeated batch in comparison to fed-batch operation is that repeated batch avoids inhibition due to the accumulation of cytotoxic compounds in the broth, as may occur with the fed-batch operation [28].…”

Section: Succinic Acid Production In a Repeated Batch Bioreactormentioning

confidence: 99%

Study on the Operational Modes Using Both Growing and Resting Cells for Succinic Acid Production from Xylose Kinetic Modelling

et al. 2023

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Succinic acid (SA) is one of the most prominent C4 biomass-based platform chemicals that can be biologically obtained. This article verifies, for the first time, the possibility of producing succinic acid with fed-batch or repeated batch operations with Actinobacillus succinogenes in a resting state, that is, in the absence of a nitrogen source. In this work it is possible to optimise separately the stages of cell growth and production in the fed-batch or repeated batch modes, minimising the costs associated with the nitrogen source and facilitating the subsequent purification of SA. These experiments were carried out with xylose, the most abundant monosaccharide in hemicelluloses, with the results subsequently being compared to those obtained in equivalent operations carried out with cells in a state of growth. First, a cost-effective synthetic growth medium was proposed and successfully employed for SA production. Biocatalysts’ reutilisation showed that the bioprocess can be carried out successfully in repeated batch and fed-batch modes. The best mode for growing cells is repeated batch, achieving a maximum productivity of 0.77 g‧L−1‧h−1, a selectivity of 53% and a yield of 51% with respect to xylose consumed. In contrast, the fed-batch mode was found to be the most convenient mode with resting cell biocatalyst, reaching a maximum productivity of 0.83 g‧L−1‧h−1, a selectivity of 0.78 g‧g−1 and a yield of 68% with respect to the xylose consumed. In addition, by-product formation is significantly reduced when employing resting cells. An unstructured non-segregated kinetic model was developed for both biocatalysts, capable of simulating cell growth, xylose consumption, SA production and by-product generation, with successful estimation of kinetic parameters supported by statistical criteria.

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Section: Succinic Acid Production In a Repeated Batch Bioreactormentioning

confidence: 99%

Study on the Operational Modes Using Both Growing and Resting Cells for Succinic Acid Production from Xylose Kinetic Modelling

et al. 2023

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“…The copyright holder for this preprint this version posted June 30, 2023. ; https://doi.org/10.1101/2023.06. 30.547221 doi: bioRxiv preprint technologies, including fed-batch culture (2,3), dialysis culture (4)(5)(6), and semicontinuous culture (7), had been developed and applied accordingly, attaining HCDC via providing additional nutrients and diluting or removing inhibitors coincidentally. However, they all require sophisticated instrumentation and some of them such as dialysis culture waste a considerable amount of nutrients, combinedly leading to high cost.…”

Section: Introductionmentioning

confidence: 99%

BiodecomposingSpirulina platensisby ade novodesignedBacillus subtilis-based method to develop a medium for the high cell-density cultivation ofEscherichia coliin batch mode

Zhang

et al. 2023

Preprint

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High cell-density cultivation (HCDC) is fundamental to basic research and industrial applications, especially in batch mode. However, limited media are available for batch culture of HCDC, because the media for batch culture must contain extremely sufficient nutrients on the one hand and few or even no substrates to generate detrimental metabolite on the other hand to attain HCDC. Spirulina platensis (SP), a new media material, is considered ideal for the development of such media. Here, we develop a biolysis method for SP degradation based on the cultivation supernatant of Bacillus subtilis and extensively demonstrate its higher degradation (indicated by the production of more small peptides and free amino acids) and cultivation effectiveness with three other methods. Based on its SP hydrolysates, a modified SP-based broth (MSP) is then formulated. Tests on Escherichia coli K-12 show that MSP achieves HCDC with several benefits: (i) its maximum optical density at 600 nm is ~16.67, significantly higher than that of Luria-Bertani (LB) broth (only ~6.30); (ii) MSP requires only 36 h to reach peak growth, much faster than that of LB (48 h); (iii) its maximum growth (1.12 ± 0.01 h−1) is significantly higher than that of LB (0.20 ± 0.00 h−1); (v) MSP initiates growth immediately after inoculation (lag time <0), comparable to LB; (iv) the number of viable cells in MSP is high (~2.16 × 1011ml-1), ~10.19 times the amount in LB. Consequently, we envision MSP will become the first choice for E. coli HCDC batch culture in the future.

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“…Lactulose was synthesized from fructose and lactose in continuous packed-bed reactor operation with glyoxyl-agarose immobilized Aspergillus oryzae β-galactosidase [ 44 ]. Lactulose synthesis with biological enzymes could overcome some disadvantages of industrial chemical synthesis, such as the degradation of lactulose, side reaction, purification of late products, and so on [ 45 ]. Lactose can be converted into galactose and glucose under the catalysis of lactase [ 46 ].…”

Section: Introductionmentioning

confidence: 99%

Co-Immobilization of Lactase and Glucose Isomerase on the Novel g-C3N4/CF Composite Carrier for Lactulose Production

et al. 2022

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The g-C3N4/CF composite carrier was prepared by ultrasound-assisted maceration and high-temperature calcination. The enzyme immobilization using the g-C3N4/CF as the novel carrier to immobilize lactase and glucose isomerase was enhanced for lactulose production. The carbon fiber (CF) was mixed with melamine powder in the mass ratio of 1:8. The g-C3N4/CF composite carrier was obtained by calcination at 550 °C for 3 h. After the analysis of characteristics, the g-C3N4/CF was successfully composited with the carbon nitride and CF, displaying the improvement of co-immobilization efficiency with the positive effects on the stability of the enzyme. The immobilization efficiency of the co-immobilized enzyme was 37% by the novel carrier of g-C3N4/CF, with the enzyme activity of 13.89 U g−1 at 60 °C. The relative activities of co-immobilized enzymes maintained much more steadily at the wider pH and higher temperature than those of the free dual enzymes, respectively. In the multi-batches of lactulose production, the relative conversion rates in enzymes co-immobilized by the composite carrier were higher than that of the free enzymes during the first four batches, as well as maintaining about a 90% relative conversation rate after the sixth batch. This study provides a novel method for the application of g-C3N4/CF in the field of immobilizing enzymes for the production of lactulose.

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Enzymatic production of lactulose by fed-batch and repeated fed-batch reactor

Cited by 16 publications

References 33 publications

Study on the Operational Modes Using Both Growing and Resting Cells for Succinic Acid Production from Xylose Kinetic Modelling

Study on the Operational Modes Using Both Growing and Resting Cells for Succinic Acid Production from Xylose Kinetic Modelling

BiodecomposingSpirulina platensisby ade novodesignedBacillus subtilis-based method to develop a medium for the high cell-density cultivation ofEscherichia coliin batch mode

Co-Immobilization of Lactase and Glucose Isomerase on the Novel g-C3N4/CF Composite Carrier for Lactulose Production

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